Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Cycloscope B-NHL

  • Product Code: X1051
  • Size: 20 Tests
  • Availability: In Stock In Stock
  • Price (USD): $682

Cat #

X1051		 Quantity:      

Data Sheet

Product Name

Cycloscope B-NHL

Product Type

Flow Cytometry Kits

Applications

Flow Cytometry

Size

20 Tests

Price (USD)

$682

Background

CYCLOSCOPE B-NHL, is a kit used for the flow cytometric analysis of DNA cell contents in B- cell non-Hodgkin?s Lymphoma (B-NHL) and B-lineage chronic lymphocytic leukemias (B-CLL). This kit is mainly focused for DNA studies of B-cells from bone marrow or peripheral blood samples of these patients. In recent years, DNA flow cytometry studies have extended from basic research to clinical laboratories. Thus, flow cytometry analysis of the distribution of the cell nuclei DNA contents is being widely used to estimate the cell cycle distribution and the existence of DNA aneuploidy of either normal and tumour cell populations. Cycloscope kits are based on the combination of DNA cell measurements together with the analysis of tumoral cell antigen expression. This double staining method allows the identification of the neoplastic cells present in the sample in order to performe a DNA analysis separately from that of normal hemopoietic cells, as it is recommended by different consensus reports on DNA analysis by flow cytometry in neoplastic hematopathology. Previous studies have demonstrated that the percentage of tumor B-cells in S-phase have a clear role as an independent prognostic factor either in non-Hodgkin`s lymphoma or in chronic lymphocytic leukemia. On the other hand, it has been shown that DNA-ploidy correlates well with histopathologic grade in non-Hodgkin?s lymphoma patients.

Customer Storage

Product should be stored at 4-8°C. DO NOT FREEZE

References

1-.Terstappen LWMM, Johnsen W, Segers-Nolten IMJ, Loken MR. Identification and Characterization of Normal Human Plasma Cells in Normal Human Bone Marow by High Resolution Flow Cytometry. Blood, 76:1739-1747 (1990).
2-.Orfao A, Ciudad J, González M, San Miguel JF, García AR, López-Berges MC, Ramos F, Del Cañizo MC, Rios A, Sanz M, López-Borrasca A. Prognostic value of S-phase white blood cell count in B-cell chronic lymphocytic leukemia. Leukemia, 6: 47-51 (1992).
3-. Duque RE, Andreeff M, Braylan RC, Diamond LW, Peiper SC. Consensus review of the clinical utility of ADN flow cytometry in neoplastic hematopathology. Cytometry, 14: 492-496 (1993).
4-. Stelzer GT, Marti G, Hurley A, McCoy P, Lovett EJ, Schwartz A. U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry, 30: 214-230 (1997).