Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Cycloscope B-NHL

  • Product Code: X1051
  • Size: 20 Tests
  • Price (USD): $682

Cat #

X1051		 Quantity:      

Data Sheet

Product Name

Cycloscope B-NHL

Product Type

Flow Cytometry Kits

Applications

Flow Cytometry

Size

20 Tests

Price (USD)

$682

Background

CYCLOSCOPE B-NHL, is a kit used for the flow cytometric analysis of DNA cell contents in B- cell non-Hodgkin's Lymphoma (B-NHL) and B-lineage chronic lymphocytic leukemias (B-CLL). This kit is mainly focused for DNA studies of B-cells from bone marrow or peripheral blood samples of these patients. In recent years, DNA flow cytometry studies have extended from basic research to clinical laboratories. Thus, flow cytometry analysis of the distribution of the cell nuclei DNA contents is being widely used to estimate the cell cycle distribution and the existence of DNA aneuploidy of either normal and tumour cell populations. Cycloscope kits are based on the combination of DNA cell measurements together with the analysis of tumoral cell antigen expression. This double staining method allows the identification of the neoplastic cells present in the sample in order to performe a DNA analysis separately from that of normal hemopoietic cells, as it is recommended by different consensus reports on DNA analysis by flow cytometry in neoplastic hematopathology. Previous studies have demonstrated that the percentage of tumor B-cells in S-phase have a clear role as an independent prognostic factor either in non-Hodgkin`s lymphoma or in chronic lymphocytic leukemia. On the other hand, it has been shown that DNA-ploidy correlates well with histopathologic grade in non-Hodgkin's lymphoma patients.

Customer Storage

Product should be stored at 4-8ºC. DO NOT FREEZE

References

1-.Terstappen LWMM, Johnsen W, Segers-Nolten IMJ, Loken MR. Identification and Characterization of Normal Human Plasma Cells in Normal Human Bone Marow by High Resolution Flow Cytometry. Blood, 76:1739-1747 (1990). 2-.Orfao A, Ciudad J, Gonza?lez M, San Miguel JF, Garci?a AR, Lo?pez-Berges MC, Ramos F, Del Can?izo MC, Rios A, Sanz M, Lo?pez-Borrasca A. Prognostic value of S-phase white blood cell count in B-cell chronic lymphocytic leukemia. Leukemia, 6: 47-51 (1992). 3-. Duque RE, Andreeff M, Braylan RC, Diamond LW, Peiper SC. Consensus review of the clinical utility of ADN flow cytometry in neoplastic hematopathology. Cytometry, 14: 492-496 (1993). 4-. Stelzer GT, Marti G, Hurley A, McCoy P, Lovett EJ, Schwartz A. U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry, 30: 214-230 (1997).