Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

14-3-3 theta/tau

  • Product Code: X1010
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $201

Cat #

X1010		 Quantity:      

Data Sheet

Product Name

14-3-3 theta/tau

Synonyms

mouse anti 14-3-3 theta/tau, theta tau

Host/Source

Mouse

Clone

3B9

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse, Rat, Bovine

Applications

Enzyme Immunoassay, Western Blot

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$201

Background

At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and, through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

Immunogen

Recombinant 14-3-3 / protein

Positive Control

3T3, PC12, Jurkat

Formulation

Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5 and 3 mM sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

31 kDa

Product Image

Image Legend

At right is a typical western blot (10% SDS-PAGE) of 3T3 (lane 1), PC12 (lane 2), MDBK (lane 3), Jurkat (lane 4) and normal human fibroblasts (lane 5).

Database Links:

SwissProtP27348Human

References

1. Umahara, T., et al. 'Structure-oriented review of 14-3-3 protein isoforms in geriatric neuroscience.' Geriatr. Gerontol. Int. 2012, epub.

2. Choi, M.R., et al. 'Effect of fluoxetine on the expression of tryptophan hydroxylase and 14-3-3 protein in the dorsal raphe nucleus and hippocampus of rat.' J. Chem. Neuronat., 2012, 43, 96-102

3. Obsil, T., et al. 'Structural basis of 14-3-3 protein function.' Semin. Cell Dev. Biol. 2011, 22, 663-672.


Product Specific References


This product has been used in:

1. Kakinuma, N., et al. ?Kank regulates RhoA-dependent formation of actin stress fibers and cell migration via 14-3-3 in PI3K?Akt signaling.? J. Cell Biol. 2008, 181, 537-549
2. Patel, A., et al. ?Host protein interactions with enteropathogenic Escherichia coli (EPEC): 14-3-3 binds Tir and has a role in EPEC-induced actin polymerization.? Cellular Microbiology, 2006, 8, 55-71