Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

14-3-3 theta/tau

  • Product Code: X1010
  • Size: 100 µg
  • Price (USD): $201

Cat #

X1010		 Quantity:      

Data Sheet

Product Name

14-3-3 theta/tau

Synonyms

mouse anti 14-3-3 theta/tau, theta tau

Host/Source

Mouse

Clone

3B9

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse, Rat, Bovine

Applications

Enzyme Immunoassay, Western Blot

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$201

Background

At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and, through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

Immunogen

Recombinant 14-3-3?/? protein

Positive Control

3T3, PC12, Jurkat

Formulation

Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5 and 3 mM sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

31 kDa

Product Image

Image Legend

At right is a typical western blot (10% SDS-PAGE) of 3T3 (lane 1), PC12 (lane 2), MDBK (lane 3), Jurkat (lane 4) and normal human fibroblasts (lane 5).

Database Links:

SwissProtP27348Human

References

1. Umahara, T., et al. 'Structure-oriented review of 14-3-3 protein isoforms in geriatric neuroscience.' Geriatr. Gerontol. Int. 2012, epub. 2. Choi, M.R., et al. 'Effect of fluoxetine on the expression of tryptophan hydroxylase and 14-3-3 protein in the dorsal raphe nucleus and hippocampus of rat.' J. Chem. Neuronat., 2012, 43, 96-102 3. Obsil, T., et al. 'Structural basis of 14-3-3 protein function.' Semin. Cell Dev. Biol. 2011, 22, 663-672.


Product Specific References


This product has been used in:

1. Kakinuma, N., et al. 'Kank regulates RhoA-dependent formation of actin stress fibers and cell migration via 14-3-3 in PI3K–Akt signaling.' J. Cell Biol. 2008, 181, 537-549 2. Patel, A., et al. 'Host protein interactions with enteropathogenic Escherichia coli (EPEC): 14-3-3? binds Tir and has a role in EPEC-induced actin polymerization.' Cellular Microbiology, 2006, 8, 55-71