Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

ADAR2 (Adenosine Deaminase Acting on RNA 2)

  • Product Code: U115P
  • Size: 50 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

U115P		 Quantity:      

Data Sheet

Product Name

ADAR2 (Adenosine Deaminase Acting on RNA 2)

Synonyms

RED-1, RED1; ADAR2, DRADA2 dsRNA adenosine deaminase, RNA-editing deaminase 1, RNA-editing enzyme 1

Host/Source

Sheep

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Rat

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

50 µg

Price (USD)

$244

Background

RED 1 also called ADAR2 is involved in the editing of the messenger RNAs for glutamate receptor (GluR) subunits by site-selective adenosine deamination. It edits both the GluR-B Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently.

Immunogen

GST fusion protein containing aa 6-66 of the rat RED1 coding sequence

Positive Control

Rat Brain

Formulation

Affinity Purified

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

80.7 kDa

Product Image

Image Legend

Immunofluorescence staining of mouse anterior horns of spinal cord at 6 months, 15 months and 26 months of age. Antibody used at 1:100 dilution and visualized using donkey anti-sheep conjugated to Alexa 555 dye (red color). The blue color is a fluorescent nuclear probe, TOPRO3. (Image modified from original in citation 1)

Database Links:

SwissProtP51400Rat

Product Specific References


This product has been used in:

1. Hideyama, T., et al. ?Co-Occurrence of TDP-43 Mislocalization with Reduced Activity of an RNA Editing Enzyme, ADAR2, in Aged Mouse Motor Neurons? PLoS One, 7, e43469, 2012
2. Yamashita, T., et al. ?Rescue of amyotrophic lateral sclerosis phenotype in a mouse model by intravenous AAV9-ADAR2 delivery to motor neurons.? EMBO Mol. Med., 2013, 5, 1710-1719