Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.


Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Disabled (NT)

  • Product Code: U100P
  • Size: 250 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

U100P		 Quantity:      

Data Sheet

Product Name

Disabled (NT)


Disabled homolog 1; DAB1





Product Type

Polyclonal Antibody




Western Blot


Ammonium Sulfate Precipitation


250 µg

Price (USD)



Disruption of the disabled-1 gene (Dab1) results in aberrant migration of neurons during development and disorganization of laminar structures throughout the brain. Dab1 is thought to function as an adaptor molecule in signal transduction processes. It contains a protein-protein interaction (PI) similar to the phosphotyrosine binding domain of the Shc oncoprotein, it is phosphorylated by the Src protein tyrosine kinase, and it binds to SH2 binding domains in a phosphotyrosine dependent manner. Disabled ?1 acts downstream of reelin in a signalling pathway.


Recombinant protein corresponding to amino acids 1-179 of mouse disabled coupled to a GST fusion protein.

Positive Control

293 cells transfected with Dab-1 HA tag


Provided as 0.2mm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight


Product Image

Image Legend

Western blot analysis using Disabled-1 (NT) (Cat. No. U100P) (1) and (CT) (Cat. No. U105P) (2) on mouse brain extracts.

Database Links:



1. Howell B.W. et.al (1997). EMBO Journal 1,121-132
2. Homayouni,R. et.al (1999) J. Neuroscience 19(17), 7505-7515

Product Specific References

This product has been used in:

1. Cuchillo-Ibanez, I., et al. ?Beta-Amyloid Impairs Reelin Signaling.? PLoS One, 8, e72297 (2013)