Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Disabled (NT)

  • Product Code: U100P
  • Size: 250 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

U100P		 Quantity:      

Data Sheet

Product Name

Disabled (NT)

Synonyms

Disabled homolog 1; DAB1

Host/Source

Sheep

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Mouse

Applications

Western Blot

Purification

Ammonium Sulfate Precipitation

Size

250 µg

Price (USD)

$244

Background

Disruption of the disabled-1 gene (Dab1) results in aberrant migration of neurons during development and disorganization of laminar structures throughout the brain. Dab1 is thought to function as an adaptor molecule in signal transduction processes. It contains a protein-protein interaction (PI) similar to the phosphotyrosine binding domain of the Shc oncoprotein, it is phosphorylated by the Src protein tyrosine kinase, and it binds to SH2 binding domains in a phosphotyrosine dependent manner. Disabled ?1 acts downstream of reelin in a signalling pathway.

Immunogen

Recombinant protein corresponding to amino acids 1-179 of mouse disabled coupled to a GST fusion protein.

Positive Control

293 cells transfected with Dab-1 HA tag

Formulation

Provided as 0.2mm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

63

Product Image

Image Legend

Western blot analysis using Disabled-1 (NT) (Cat. No. U100P) (1) and (CT) (Cat. No. U105P) (2) on mouse brain extracts.

Database Links:

SwissProtP97318Mouse

References

1. Howell B.W. et.al (1997). EMBO Journal 1,121-132
2. Homayouni,R. et.al (1999) J. Neuroscience 19(17), 7505-7515


Product Specific References


This product has been used in:

1. Cuchillo-Ibanez, I., et al. ?Beta-Amyloid Impairs Reelin Signaling.? PLoS One, 8, e72297 (2013)