Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Alx-4

  • Product Code: T110M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $232

Cat #

T110M		 Quantity:      

Data Sheet

Product Name

Alx-4

Synonyms

mAlx4, mouse anti mouse Alx-4

Host/Source

Mouse

Clone

KAbB4

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Mouse

Applications

Western Blot

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$232

Background

Alx-4 is a transcriptional activation factor whose expression is restricted to sites of epithelial mesenchymal interactions. Alx-4 is a novel paired-likehomeodomain protein. Alx-4 is expressed on a variety of tissues including; osteoblast precursers, dermal papilla of hair and whisker follicles, dental papilla of teeth and mesenhymal cells.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant Alx-4 protein and mouse myeloma cells.

Positive Control

Recombinant Alx-4 protein

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

42.8

Database Links:

SwissProtO35137Human

References

1. Rendl, Michael et al., 'Molecular Dissection of Mesenchymal-Epithelial Interactions in the Hair Follicle' Public Library of Science 2005, 3, 11, e331


Product Specific References


This product has been used in:

1. Rendl, Michael et al., 'Molecular Dissection of Mesenchymal-Epithelial Interactions in the Hair Follicle' Public Library of Science 2005, 3, 11, e331