Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.


Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

bax (universal)

  • Product Code: A105M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

A105M		 Quantity:      

Data Sheet

Product Name

bax (universal)









Product Type

Monoclonal Antibody


Human, Mouse, Rat




Protein A/G Chromatography


100 µg

Price (USD)



The immunogen for the anti-universal bax was a synthetic peptide corresponding to amino acids 12 to 24 of the human bax sequence. Bax expression is associated with cell death regulation. The ratio of bax to other bcl-2 family members is believed to regulate apoptosis. Clone 6A7 is conformation dependant and will recognize only detergent treated bax protein and not the native form.


A synthetic peptide corresponding to amino acids 12 to 24 of the human bax sequence


Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight


Database Links:



1. Y.T. Hsu et. al. (1997) J. Biol. Chem., 272(21): 13829-13834

2. Y. T. Hsu et. al (1997) Proc. Natl. Acad. Sci. USA, 94: 3668-3672

3. Smoot, R.L., et al (2010) Cancer Res., 70, 1960-1969

Product Specific References

This product has been used in:

1. Lau, E., et al. ?PKC regulates ATF2 availability to alter mitochondrial permeability following genotoxic stress.? Cell, 148, 543-555 (2012)
2. Cazanave, S.C., et. ?JNK1-dependent PUMA Expression Contributes to
Hepatocyte Lipoapoptosisal.?
3. Akazawa, Y., et al. ?Endoplasmic Reticulum Stress Contributes to Helicobacter Pylori VacA-Induced Apoptosis.? PLoS One, 8, e82322 (2013)