Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

bax (universal)

  • Product Code: A105M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

A105M		 Quantity:      

Data Sheet

Product Name

bax (universal)

Synonyms

ubax

Host/Source

Mouse

Clone

6A7

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$244

Background

The immunogen for the anti-universal bax was a synthetic peptide corresponding to amino acids 12 to 24 of the human bax sequence. Bax expression is associated with cell death regulation. The ratio of bax to other bcl-2 family members is believed to regulate apoptosis. Clone 6A7 is conformation dependant and will recognize only detergent treated bax protein and not the native form.

Immunogen

A synthetic peptide corresponding to amino acids 12 to 24 of the human bax sequence

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

21.2

Database Links:

SwissProtQ07812Human

References

1. Y.T. Hsu et. al. (1997) J. Biol. Chem., 272(21): 13829-13834

2. Y. T. Hsu et. al (1997) Proc. Natl. Acad. Sci. USA, 94: 3668-3672

3. Smoot, R.L., et al (2010) Cancer Res., 70, 1960-1969


Product Specific References


This product has been used in:

1. Lau, E., et al. ?PKC regulates ATF2 availability to alter mitochondrial permeability following genotoxic stress.? Cell, 148, 543-555 (2012)
2. Cazanave, S.C., et. ?JNK1-dependent PUMA Expression Contributes to
Hepatocyte Lipoapoptosisal.?
3. Akazawa, Y., et al. ?Endoplasmic Reticulum Stress Contributes to Helicobacter Pylori VacA-Induced Apoptosis.? PLoS One, 8, e82322 (2013)