Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

CD42a PE

  • Product Code: 42A4
  • Size: 100 Tests
  • Availability: In Stock In Stock
  • Price (USD): $191

Cat #

42A4		 Quantity:      

Data Sheet

Product Name

CD42a PE

Synonyms

CD42a PE

Host/Source

Mouse

Clone

ESS

Isotype

IgG1

Product Type

Single-Color Reagent

Reactivity

Human

Applications

Flow Cytometry

Purification

Protein A/G Chromatography

Size

100 Tests

Price (USD)

$191

Background

Single-chain membrane glycoprotein that forms a non-covalent complex with GPIb. (MW23kDa) Reactivity with resting and activated platelets, weakly on monocytes, megakaryocytes and attachment site for the platelet plasma membrane to the submembrane cytoskeleton. GPIb/IX complex, functions as the receptor for ristocetin-induced binding of von Willebrand factor and as the von Willebrand factor-depend adhesion receptor.

Formulation

Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Customer Storage

Product should be stored at 4-8°C. DO NOT FREEZE

Database Links:

SwissProtP14770Human

References

1. Detection of platelet activation with monoclonal antibodies and flow cytometry. Fijnheer R., Modderman P.W., Veldman H., et al. Transfusion 1990:30:20-25

2. Glycoprotein Ib and glycoprotein IX are fully complexed in the intact platelet membrane Du X, Beutler L., Ruan C., Castaldi P.A., and Berndt M.C., Blood 1987:69:1524-1527

3. Structure of the glycoprotein Ib-IX complex from platelet membranes Fox JEB, Aggerbeck L.P., Berndt M.C., J. Biol. Chem. 1988:263:4882-4890

4. Leucocyte Typing IV: White Cell Differentiation Antigens Nieuwenhuis H.K. Report on functional studies. In: Knapp W., Dorken B., Gilks W.R., et al, eds. Oxford: Oxford University Press: 1989:1002-1003.


Product Specific References


This product has been used in:

1. Heilmann, C., et al, 'Staphylococcus aureus Fibronectin-Binding Protein (FnBP)?Mediated Adherence to Platelets, and Aggregation of Platelets Induced by FnBPA but Not by FnBPB' Journal of Infectious Disease 2004, 190, , 321-329