Catalogue

Background
CD45RA recognizes Mr. 220-kdalton of the leucocyte antigen (LCA). CD45RA antigen is a member of the CD45 antigen family that also includes CD45, CD45RO, and CD45RB antigens. CD45RA is expressed on approximately 75% of CD8 lymphocytes and 50% of CD4 lymphocytes, all B cells and NK Cells. CD45RA antigen is expressed on naive T lymphocytes, antigen density decreases upon in vitro activation. A decrease antigen density has been demonstrated for CD4+CD45RA+subset with active multiple sclerosis.

Synonyms: CD45RA FITC

Source

Immunogen: Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient

Product

Product Form: FITC

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Purification Method: Protein A/G Chromatography

Concentration: Titered for flow cytometry

Applications
Add 10 µl of MAB / 100 µl of 10^6 PBMC in PBS. Mix Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. Add 10 µl of MAB / 100 µl of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse Whole Blood and analyze or fix with 0.5% v/v paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at 4-8°C. DO NOT FREEZE

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Leucocyte Typing IV: White Cell Differentiation Antigens, Schwinzer R. Cluster report: CD45/CD45R, Knapp W., Dorken B., Gilks W.R.,et al, eds. Oxford University Press; 1989:628-634 2. CD45 isoform expression on human neonatal T cells: expression and turnover of CD45 isoforms on neonatal versus adult T cells after activation. Cell Immunol 1992 Jun;142(1):114-24 Yamada A; Kaneyuki T; Hara A; Rothstein DM; Yokoyama MM 3. Transient accumulation and subsequent rapid loss of messenger RNA encoding high molecular mass CD45 isoforms after T cell activation. J Immunol 1992 Mar 15;148(6):1898-905 Deans JP; Serra HM; Shaw J; Shen YJ; Torres RM; Pilarski LM 4. Patterns of membrane CD45 isoform expression by leukaemic blasts and normal mature myeloid cells. Int J Hematol 1992 Jun;55(3):235-42 Master PS; Richards SJ; Roberts BE; Scott CS 5. CD45 isoform expression during T cell development in the thymus Eur J Immunol 1992 Jul;22(7):1843-50 Fujii Y; Okumura M; Inada K; Nakahara K; Matsuda H 6. Functional subsets of T cells defined by isoforms of CD45 Biochem Soc Trans 1992 Feb;20(1):184-7 Beverley PC; Daser A; Michie CA; Wallace DL

Protein Reference(s)

Database Name: UniProt

Accession Number: P08575

Safety Datasheet(s) for this product: