Total cell Iysates are useful as both positive and negative controls in immunoblotting. A431, a human epidermoid carcinoma cell line, and the EGF-stimulated A431 Iysates are used as negative and positive controls, respectively, when studying the phosphorylation cascade initiated by ligand binding to receptor tyrosine kinases. Both A431 and normal human fibroblasts are valuable positive controls, for antibodies raised against proteins expressed in these cells. The mouse fibroblast cell line 3T3 and its RSV transformed counterpart serve as negative and positive controls, respectively, for antibodies specific for proteins that are phosphorylated as a result of transformation by RSV (Rous Sarcoma Virus). Madin-Darby bovine kidney (MDBK) cell Iysate displays proteins expressed in bovine kidney, and PC12 (rat phaeochromocytoma) cell Iysate can be expected to mirror the protein expression of cells and tissues of neuroectodermal origin. Likewise, Jurkat cells express the proteins unique to human T-cells. Rat brain is a widely used positive control for those proteins that are exclusively expressed in brain.

Code Name Product Type Clone
X1005 3T3 cells Control Lysate
X1002 A431 cells Control Lysate
X1003 A431 cells stimulated with EGF Control Lysate
X1487C EDG8 (S1P5) Control Lysate
X1004 Fibroblasts Control Lysate
X1278 HeLa Control Lysate
X1633C Human Brain Tissue Control Lysate
X1634C Human Heart Tissue Control Lysate
X1635C Human Placenta Tissue Control Lysate
X1009 Jurkat cells Control Lysate
X1007 kidney (MDBK) cells Control Lysate
X1218C Lysophosphatidic Acid Receptor 1 (EDG2) Control Lysate
X1219C Lysophosphatidic Acid Receptor 2 (EDG-4) Control Lysate
X1220C Lysophosphatidic Acid Receptor 3 (EDG-7) Control Lysate
X1601C OGR1/GPR68 Control Lysate
X1008 PC12 (rat phaeochromocytoma) cells Control Lysate
X1030 Rat Brain Control Lysate
X2392C RMS13 Control Lysate
X1217C Sphingosine 1 Phosphate Receptor 1 (EDG-1) Control Lysate
X1311C Sphingosine 1 Phosphate 4 Receptor (CT) (EDG6) Control Lysate
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