Control antibodies are a vital part of assay design and ensure signal detected during antibody-based applications, can be attributed to specific antigen binding, rather than non-specific background staining.
Negative isotype controls function by determining the level of non-specific binding of the primary antibody to target. This is achieved by matching the control antibody host species, isotype, and if applicable fluorochrome, to that of the experimental specific antibody. A matched fluorochrome is required if the experimental primary antibody is directly conjugated. Negative control antibody is deliberately generated against a chemical or target not expressed by the cell or tissue of interest, so any binding will be non-specific in nature. This non-specific binding is most frequently caused by Fc receptors expressed on the surface of the target sample. These receptors bind the antibody’s Fc region independently of their Fab epitope binding domains.
Control antibodies are most commonly used in flow cytometry or immunohistochemistry to determine levels of background staining, but can also be utilized as blocking agents before addition of primary antibody.
|BIgG||Bovine IgG||Isotype control|
|GIC-201||COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE)||Negative Control||VI-AP and VI-AP|
|GCT-202||COMBI Surface: IgG2a Negative Control (FITC) and IgG1 Negative Control (PE)||Negative Control||4H1-A7 and VI-AP|
|GIgG||Goat IgG||Isotype control|
|GIgG/FITC||Goat IgG, conjugated with FITC|
|GIgG/PO||Goat IgG, conjugated with Horseradish peroxidase|
|GpIgG||Guinea Pig IgG||Isotype control|
|0G11||IgG1||Second Step Reagents||ZX3|
|X1065||IgG1 Cy5-PE||Single-Color Negative Control||ZX3|
|GM-4991||IgG1 Negative Control||Negative Control||VI-AP|
|GM-4992||IgG1 Negative Control, conjugated to FITC||Negative Control||VI-AP|
|GM-4993||IgG1 Negative Control, conjugated to PE||Negative Control||VI-AP|
|02A1||IgG2a||Single-Color Negative Control|
|X1049||IgG2a / IgG2b Cy5RPE||Single-Color Negative Control|
|MonIgG||Monkey (Rhesus) IgG||Isotype control|
|RIgG||Rabbit IgG||Isotype control|
|RIgG/FITC||Rabbit IgG, conjugated with FITC|
|RIgG/PO||Rabbit IgG, conjugated with Horseradish peroxidase|
|RaIgG||Rat IgG||Isotype control|
|ShIgG||Sheep IgG||Isotype control|
|SwIgG||Swine IgG||Isotype control|