Accelerating the Pace of Discovery

Control antibodies

Control antibodies are a vital part of assay design and ensure signal detected during antibody-based applications, can be attributed to specific antigen binding, rather than non-specific background staining.

Negative isotype controls function by determining the level of non-specific binding of the primary antibody to target. This is achieved by matching the control antibody host species, isotype, and if applicable fluorochrome, to that of the experimental specific antibody. A matched fluorochrome is required if the experimental primary antibody is directly conjugated. Negative control antibody is deliberately generated against a chemical or target not expressed by the cell or tissue of interest, so any binding will be non-specific in nature. This non-specific binding is most frequently caused by Fc receptors expressed on the surface of the target sample. These receptors bind the antibody’s Fc region independently of their Fab epitope binding domains.

Control antibodies are most commonly used in flow cytometry or immunohistochemistry to determine levels of background staining, but can also be utilized as blocking agents before addition of primary antibody.

Code Name Product Type Clone
02A2 Mouse IgG2a FITC Negative Control Single-Color Negative Control
0G11 Mouse IgG1 Negative Control Second Step Reagents ZX3
BIgG Bovine IgG Isotype control
DIgY-010 Normal Duck IgY (egg-derived) Control antibodies
DIgY-100 Normal Duck IgY (egg-derived) Control antibodies
DIgY-1000 Normal Duck IgY (egg-derived) Control antibodies
DIgY-B Normal Duck IgY (egg-derived), conjugated with Biotin Control antibodies
DIgY-F Normal Duck IgY (egg-derived), conjugated with FITC Control antibodies
DIgY-HRP Normal Duck IgY (egg-derived), conjugated with HRP Control antibodies
MonIgG Monkey (Rhesus) IgG Isotype control
GIC-201 COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE) Negative Control VI-AP and VI-AP
OIgY-100 Normal Ostrich IgY (egg-derived) Control antibodies
OIgY-010 Normal Ostrich IgY (egg-derived) Control antibodies
OIgY-1000 Normal Ostrich IgY (egg-derived) Control antibodies
OIgY-B Normal Ostrich IgY (egg-derived), conjugated with Biotin Control antibodies
OIgY-F Normal Ostrich IgY (egg-derived), conjugated with FITC Control antibodies
OIgY-HRP Normal Ostrich IgY (egg-derived), conjugated with HRP Control antibodies
QuIgY-010 Normal Quail IgY (egg-derived) Control antibodies
QuIgY-100 Normal Quail IgY (egg-derived) Control antibodies
QuIgY-1000 Normal Quail IgY (egg-derived) Control antibodies
QuIgY-B Normal Quail IgY (egg-derived), conjugated with Biotin Control antibodies
QuIgY-F Normal Quail IgY (egg-derived), conjugated with FITC Control antibodies
QuIgY-HRP Normal Quail IgY (egg-derived), conjugated with HRP Control antibodies
RIgG Rabbit IgG Isotype control
RIgG/FITC Rabbit IgG, conjugated with FITC
RIgG/PO Rabbit IgG, conjugated with Horseradish peroxidase
SwIgG Swine IgG Isotype control
ShIgG Sheep IgG Isotype control
TIgY-010 Normal Turkey IgY (egg-derived) Control antibodies
TIgY-100 Normal Turkey IgY (egg-derived) Control antibodies
TIgY-1000 Normal Turkey IgY (egg-derived) Control antibodies
TIgY-F Normal Turkey IgY (egg-derived), conjugated with FITC Control antibodies
TIgY-HRP Normal Turkey IgY (egg-derived), conjugated with HRP Control antibodies
TIgY-B Normal Turkey IgY (egg-derived), conjugated with Biotin Control antibodies
GIgG Goat IgG Isotype Control
GIgG/FITC Goat IgG, conjugated with FITC
GIgG/PO Goat IgG, conjugated with Horseradish peroxidase
GIgY-010 Normal Goose IgY (egg-derived) Control antibodies
GIgY-1000 Normal Goose IgY (egg-derived) Control antibodies
GIgY-B Normal Goose IgY (egg-derived), conjugated with Biotin Control antibodies
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