Von-Willebrand-FactorCatalogue number: VW500
The antibody detects normal endothelia, megakaryocytes and platelets, but also tumours derived from these cells. It is a good marker for vascularisation of tissues and re-vascularisation after injury. The antibody may also be used for the detection of tumour invasion into blood vessels. Von Willebrand factor is a co-factor (factor VIII associated antigen) of blood agglutination. It is synthesized by megakaryocytes and is released into the blood during disaggregation of thrombocytes. Additionally von Willebrand factor is built in endothelial cells and by this it becomes an important marker of these cells in immunohistochemistry. Human von-Willebrand-Factor, Factor VIII related Antigen.
Immunogen: Purified von-Willebrand-Factor isolated from human plasma
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Human
Incubation Time: 60 min at RT
Working Concentration: (RTU) neat
Pre-Treatment: Pre-treatment with pronase 0.1% (Art. No. DE110).
Positive Control: Appendix
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Fino A. (1961) Die Gerinnungsfaktoren bei der Hämophilie, Benno Schwarzenberg Verlag, Basel. 2. Sherry S. and Scriabine A. (1974) Platelets and Thrombosis , Urban & Schwarzenberg , München. 3. Hruban R.H., Kuhajda F.P., Mann R.B. (1987) Acute myelofibrosis. Immunohistochemical study of four cases and comparison with acute megakaryocytic leukemia. Am. J. Pathol. 88; 578-588.
Database Name: UniProt
Accession number: P04275 (VWF_HUMAN)
Species Accession: Human