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Von-Willebrand-Factor


Catalogue number: VW500

CloneF8/86
IsotypeIgG1
Units5 ml
HostMouse
Application IHC(C
P)
Background
The antibody detects normal endothelia, megakaryocytes and platelets, but also tumours derived from these cells. It is a good marker for vascularisation of tissues and re-vascularisation after injury. The antibody may also be used for the detection of tumour invasion into blood vessels. Von Willebrand factor is a co-factor (factor VIII associated antigen) of blood agglutination. It is synthesized by megakaryocytes and is released into the blood during disaggregation of thrombocytes. Additionally von Willebrand factor is built in endothelial cells and by this it becomes an important marker of these cells in immunohistochemistry. Human von-Willebrand-Factor, Factor VIII related Antigen.

Positive Control
Appendix

Pre-Treatment
Pre-treatment with pronase 0.1% (Art. No. DE110).

Immunogen
Purified von-Willebrand-Factor isolated from human plasma

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Working Concentration
(RTU) neat

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Applications
IHC(C, P)

Cross Reactivity
Human

Storage
2-8°C

References
1. Fino A. (1961) Die Gerinnungsfaktoren bei der Hämophilie, Benno Schwarzenberg Verlag, Basel. 2. Sherry S. and Scriabine A. (1974) Platelets and Thrombosis , Urban & Schwarzenberg , München. 3. Hruban R.H., Kuhajda F.P., Mann R.B. (1987) Acute myelofibrosis. Immunohistochemical study of four cases and comparison with acute megakaryocytic leukemia. Am. J. Pathol. 88; 578-588.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

Von-Willebrand-Factor

$242.00

SKU: VW500 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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