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VEGF-A, murine


Catalogue number: VEGA03A

Units50 µg
HostRabbit
Application ELISA
IHC(C
P)
WB
Western Blotting
Background
VEGF-A is exprimed as 4 isoforms, the smaller ones (VEGF 121 and VEGF 165) are secretory proteins, while the larger forms (VEGF 189 and VEGF 206) remain associated with the cell walls. VEGFs (Vascular Endothelial Growth Factors) are a group of homodimer glycoproteins. The subunits are linked by disulfide bonds. (MW reduced form 18-24 kDa). VEGFs are involved in angiogenesis during embryogenesis and in tumour progression and generation of metastasis. They possess mitogenic characteristics and specifically increase the permeability of endothelia in blood vessels. VEGF-A isoforms in mouse and humans (Western Blot under reducing and non-reducing conditions).

Positive Control
Metastasing tumours of different origin

Pre-Treatment
Formaldehyde fixed tissue shows good results after pre-treatment with proteinase K (0.01%, 15 min at RT), other proteases and micro-wave techniques are not validated.

Immunogen
Highly purified recombinant murine VEGF164

Purification Method
Peptide affinity purified using murine VEGF164 as matrix. Lyophilised from PBS pH 7.4 without preservative.

Working Concentration
(lyophilisate) Direct ELISA 0.5-5 µg/ml, Western Blot 0.1-0.2 µg/ml, IHC 0.5-2 µg/ml

Concentration
50 µg

Product
Peptide affinity purified using murine VEGF164 as matrix. Lyophilised from PBS pH 7.4 without preservative.

Applications
IHC(C, P), ELISA, WB

Cross Reactivity
Human, Mouse

Storage
-20°C

References


Caution
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Safety Datasheet(s) for this product:

VEGF-A, murine

$452.00

SKU: VEGA03A Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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