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Uroplakin III

Catalogue number: UP3001

CloneAU1
IsotypeIgG1
Units50 µg
HostMouse
Application IHC (P)
IHC in cryostat sections may require formaldehyde fixation
Western Blotting

Background
Together with the Uroplakins UP Ia, UP Ib and UP II, UP III is a constituent of the Asymmetric Unit Membrane of urothelial Umbrella cells. It is a 47 kDa glycoprotein, which is thought to be a specific marker for differentiated urothelia. UP III is found in surface membranes of urothelial cells of the kidney, bladder and urinary collection tract. In paraffin sections app. 60% of transitional cell carcinoma stain focally with Uroplakin III antibodies. Yet no Uroplakin has been found in non-urothelian carcinoma. Therefore it seems to be a specific marker of urothelial cells in metastases of unknown origin of the primary tumours. Uroplakin III membrane protein.

Source

Immunogen: Asymmetric Unit Membrane (AUM) preparation from bovine bladder

Product
Protein A purified antibody lyophilized from PBS containing 0.09% NaN3 and 0.5% BSA after reconstitution with 1 ml distilled water

Purification Method: Protein A purified antibody lyophilized from PBS containing 0.09% NaN3 and 0.5% BSA after reconstitution with 1 ml distilled water

Concentration: 50 µg/ml after reconstitution in 1 ml distilled water

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Human, cattle, rat, pig

Applications
IHC (P), IHC in cryostat sections may require formaldehyde fixation, WB

Incubation Time: 60 min at RT or 18 hr at 2-8°C

Working Concentration: (lyophilisate) 1-5 µg/ml

Pre-Treatment: Pre-treatment of paraffin sections with proteinase K or trypsin

Positive Control: Urothel

Storage
2-8°C

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

References
1. Moll R, Wu XR, Lin JH, Sun TT (1995) Uroplakins, specific membrane proteins of urothelial umbrella cells, as histological markers of metastatic trasitional cell carcinomas. Am. J. Pathol. 147; 1383-1397. 2. Romith R, Jezernik K, Maera A (1998) Uroplakins and cytokeratins in the regenerating rat urothelium after sodium saccharin treatment. Histochem Cell Biol 109:263-269. 3. Kaufmann O, Volmering J, and Dietel M. (2000) Uroplakin III is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinoma. Am. J. Clin. Pathol. 113; 683-687. 4. Ramos-Vara JA, Miller MA, Boucher M, Roudabush A, and Johnson GC. (2003) Immunohistochemical detection of uroplakin III, cytokeratin 7, and cytokeratin 20 in canine urothelial tumors. Vet. Pathol. 40(1); 55-62.


Uroplakin III

$774.00

SKU: UP3001 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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