Uroplakin IIICatalogue number: UP3001
IHC in cryostat sections may require formaldehyde fixation
Together with the Uroplakins UP Ia, UP Ib and UP II, UP III is a constituent of the Asymmetric Unit Membrane of urothelial Umbrella cells. It is a 47 kDa glycoprotein, which is thought to be a specific marker for differentiated urothelia. UP III is found in surface membranes of urothelial cells of the kidney, bladder and urinary collection tract. In paraffin sections app. 60% of transitional cell carcinoma stain focally with Uroplakin III antibodies. Yet no Uroplakin has been found in non-urothelian carcinoma. Therefore it seems to be a specific marker of urothelial cells in metastases of unknown origin of the primary tumours. Uroplakin III membrane protein.
Immunogen: Asymmetric Unit Membrane (AUM) preparation from bovine bladder
Protein A purified antibody lyophilized from PBS containing 0.09% NaN3 and 0.5% BSA after reconstitution with 1 ml distilled water
Purification Method: Protein A purified antibody lyophilized from PBS containing 0.09% NaN3 and 0.5% BSA after reconstitution with 1 ml distilled water
Concentration: 50 µg/ml after reconstitution in 1 ml distilled water
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Human, cattle, rat, pig
IHC (P), IHC in cryostat sections may require formaldehyde fixation, WB
Incubation Time: 60 min at RT or 18 hr at 2-8°C
Working Concentration: (lyophilisate) 1-5 µg/ml
Pre-Treatment: Pre-treatment of paraffin sections with proteinase K or trypsin
Positive Control: Urothel
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Moll R, Wu XR, Lin JH, Sun TT (1995) Uroplakins, specific membrane proteins of urothelial umbrella cells, as histological markers of metastatic trasitional cell carcinomas. Am. J. Pathol. 147; 1383-1397. 2. Romith R, Jezernik K, Maera A (1998) Uroplakins and cytokeratins in the regenerating rat urothelium after sodium saccharin treatment. Histochem Cell Biol 109:263-269. 3. Kaufmann O, Volmering J, and Dietel M. (2000) Uroplakin III is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinoma. Am. J. Clin. Pathol. 113; 683-687. 4. Ramos-Vara JA, Miller MA, Boucher M, Roudabush A, and Johnson GC. (2003) Immunohistochemical detection of uroplakin III, cytokeratin 7, and cytokeratin 20 in canine urothelial tumors. Vet. Pathol. 40(1); 55-62.