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Unmasking Fluid P ( 0.1% Pronase)


Catalogue number: DE110

Units5 ml
Application IHC(P)
Background
One of the most popular methods for antigen unmasking following formaldehyde fixation is 0.1% Pronase. It is easy to use and often preferred since it does not require high temperature. Good results have been obtained for instance with Collagen type IV, Cytokeratins, EpCAM (HEA 125), IgG, IgM, Kappa- and Lambda-chains, LCA, MAC 387, Melanoma (HMB45), Neurofilaments, p170 Glycoprotein, S-100, T-cells (CD3), Vim 3B4, Von-Willebrand-Factor. Aldehyde fixation (Formaldehyde fixation) of tissues generally causes cross-linkage of protein chains and results in masking of antigens present in the treated tissue. This makes the antigen epitopes undetectable for many antibodies applied for immunohistochemistry. Mainly monoclonal antibodies, but also mono-specific polyclonal antibodies will react with reduced binding or no binding at all. Since Formaldehyde fixation is the most important fixation method in routine histology, high temperature unmasking methods for immunohistochemistry, as developed during recent years, are very important tools for cell and tissue research and pathology.

Positive Control
S-100, Pan-Cytokeratin, Macrophages (MAC387)

Purification Method
5 ml ready to use solution of stabilised Pronase (0,1%) in dropper bottle

Working Concentration
(RTU) neat

Product
5 ml ready to use solution of stabilised Pronase (0,1%) in dropper bottle

Applications
IHC(P)

Storage
2-8°C

References


Caution
These Unmasking Fluids are manufactured for research use only. They are not intended for clinical diagnostic applications. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Unmasking Fluid P ( 0.1% Pronase)

$40.00

SKU: DE110 Categories: ,