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Unmasking Fluid H, Urea based

Catalogue number: DE008

Units250 ml
Application IHC(P)
Unmasking fluid H (urea based) has been proven to be a very helpful tool in the detection of some antigens, which are otherwise more difficult to detect. Especially good results were obtained with E-Cadherin. Aldehyde fixation (Formaldehyde fixation) of tissues generally causes cross-linkage of protein chains and results in masking of antigens present in the treated tissue. This makes the antigen epitopes undetectable for many antibodies applied for immunohistochemistry. Mainly monoclonal antibodies, but also mono-specific polyclonal antibodies will react with reduced binding or no binding at all. Since Formaldehyde fixation is the most important fixation method in routine histology, high temperature unmasking methods for immunohistochemistry, as developed during recent years, are very important tools for cell and tissue research and pathology.

Positive Control
E-Cadherin, ECM proteins and steroid receptors (e.g. Progesteron Receptor)

Purification Method
250 ml ready to use solution

Working Concentration
(RTU) neat

250 ml ready to use solution




These Unmasking Fluids are manufactured for research use only. They are not intended for clinical diagnostic applications.

Safety Datasheet(s) for this product:

Unmasking Fluid H, Urea based


SKU: DE008 Categories: ,