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Unmasking Fluid G, Glycol based


Catalogue number: DE007

Units250 ml
Application IHC(P)
Background
One of the most effective solutions for antigen unmasking is an unmasking fluid on glycol basis, one of which is our "Unmasking Fluid G" (Code No. DE007). Unmasking Fluid G makes antigens detectable after formaldehyde fixation of tissues, even after prolonged fixation times. Usage of this Unmasking Fluid G usually guarantees also a good preservation of cellular structures. In this respect it is superior to unmasking fluids on citrate or urea basis, which might have their own advantages in detection of certain antigens. Aldehyde fixation (Formaldehyde fixation) of tissues generally causes cross-linkage of protein chains and results in masking of antigens present in the treated tissue. This makes the antigen epitopes undetectable for many antibodies applied for immunohistochemistry. Mainly monoclonal antibodies, but also mono-specific polyclonal antibodies will react with reduced binding or no binding at all. Since Formaldehyde fixation is the most important fixation method in routine histology, high temperature unmasking methods for immunohistochemistry, as developed during recent years, are very important tools for cell and tissue research and pathology.

Positive Control
Human: AMACR, ATF-2 (Thr71), B cells (CD20), BAX, bcl-2 oncoprotein, CD34 (QBEND10), CEA, Cyto­keratins, c-erbB-2, Desmin, HEA125, Ki-67, (MAC387), MAP-Kinase (1/2), Melan-A (MART-1) neurofilaments, NSE, Estrogen Receptor, p53 protein, p63 protein, p170 glycoprotein, P504S, PSA, Synaptophysin, Thyroglobulin, TTF-1, T cells (CD3e and CD45RO), Vimentin (V9 and 3B4), von Willebrand-Factor etc. Rat: CD4 (W3/25), CD8 (OX-8), CD11b (OX-42), CD25 (OX-39), CD45RA (OX-33), Macrophages (ED1, ED2, MAC387)

Purification Method
250 ml 4-times concentrated solution

Working Concentration
(4 x concentrated solution) 1:4

Product
250 ml 4-times concentrated solution

Applications
IHC(P)

Storage
2-25°C

References


Caution
These Unmasking Fluids are manufactured for research use only. They are not intended for clinical diagnostic applications.

Safety Datasheet(s) for this product:

Unmasking Fluid G, Glycol based

$71.00

SKU: DE007 Categories: ,