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Unmasking Fluid C, Citrate based, pH 6


Catalogue number: DE000-1

Units1 liter
Application IHC(P)
Background
One of the routine solutions for antigen unmasking is an unmasking fluid on citrate basis, Unmasking Fluid C (Art. No. DE000 and DE000-1) Aldehyde fixation (Formaldehyde fixation) of tissues generally causes cross-linkage of protein chains and results in masking of antigens present in the treated tissue. This makes the antigen epitopes undetectable for many antibodies applied for immunohistochemistry. Mainly monoclonal antibodies, but also mono-specific polyclonal antibodies will react with reduced binding or no binding at all. Since Formaldehyde fixation is the most important fixation method in routine histology, high temperature unmasking methods for immunohistochemistry, as developed during recent years, are very important tools for cell and tissue research and pathology.

Positive Control
CEA, most Cytokeratins, Desmin, Ki-67, MAC387, Oestrogen Receptor and many others

Purification Method
1000 ml 10-times concentrated solution

Working Concentration
(10 x concentrated solution) 1:10

Product
1000 ml 10-times concentrated solution

Applications
IHC(P)

Storage
2-8°C

References


Caution
These Unmasking Fluids are manufactured for research use only. They are not intended for clinical diagnostic applications.

Unmasking Fluid C, Citrate based, pH 6

$95.00

SKU: DE000-1 Categories: ,