Rabbit anti Human UEV1A
Catalogue number: X1829P
|Product Type||Polyclonal Antibody|
Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity (1). There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).
Postive Control: Jurkat Cell Lysate. UE1A is one of seven named isoforms of UEV1. UEV1A is located in the nucleus, while iosforms 3 and 5 are found in the membrane7
Rabbit polyclonal UEV1A antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UEV1A.
Antigen Immunoaffiinity Purification
See vial for concentration
Provided as solution in phosphate buffered saline with 0.02% sodium azide
UEV1A antibody can be used for detection of UEV1A by Western blot at 1 to 2 ug/ml. Anti-UEV1A may also recognize other isoforms of UEV1A.
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Ship at ambient temperature, freeze upon arrival
Products are stable for one year from purchase when stored properly
1) Sancho E, Vila MR, Sanchez-Pulido L, et al. Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating enzymes, in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells.Mol. Cell Biol. 1998; 18:576-89. 2) Akira S and Takeda K. Toll-like receptor Signalling.Nat. Rev. Immunol. 2004; 4:499-511 3) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression.Mol. Interv. 2003; 3:466-77 4) Deng L, Wang C, Spencer E, et al. Activation of the IκB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain. Cell 2000; 103:351-61. 5) Wang C, Deng L, Hong M, et al. TAK1 is a ubiquitindependent kinase of MKK and IKK. Nature 2001; 412:346-51. 6) Yamaguchi K, Shirakabe K, Shibuya H, et al. Identification of a member of the MAPKKK family as a potential mediator of TGF-β signal transduction. Science 1995; 270:2008-11. 7) Alternative product of entry: Q13404, http://www.expasy.org/uniprot/Q13404-2, Accessed Febraury 27, 2007
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Safety Datasheet(s) for this product:
Rabbit anti Human UEV1A