TTF-1 (Thyroid Transcription Factor 1), Homeobox protein Nkx-2.1Catalogue number: TT100
The antibody to TTF-1 is well suited for the detection of the nuclear protein in thyroid gland epithelia and in lung epithelia. TTF-1 is typically found in primary lung adenocarcinoma and in Small Cell Lung Carcinoma (SCLC).The antibody doesn't react with mamma carcinoma and colon carcinoma (1,2). Therefore the antibody can be used as an adjunct for differential diagnosis of lung carcinoma. TTF-1 is a member of the NKx2 family of transcription factors. TTF1 is encoded by Homöobox-Gen NK2 homeobox 1 (NKX2-1). It is exprimed by cell nuclei of epithelial cells of the thyroid glands and the lung. It is usually not found in cell nuclei of the liver, stomach, pancreas, intestine, kidney, breast, skin, testis, pituitary, and prostate as well as inter-renal and adrenal glands (1). Thyroid Transcription Factor-1 (TTF-1), 38.5 kDa nuclear protein.
Immunogen: Recombinant rat TTF-1
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for 100-500 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for 100-500 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Concentration: 40 µg/ml
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Rat, Mouse, Human
Incubation Time: 60 min at RT
Working Concentration: (liquid conc.) 1:10 - 1:50
Pre-Treatment: For pre-treatment we recommend Biologo's Unmasking Fluid G on glycol basis (Art. No. DE007) at high temperature.
Positive Control: Thyroid or Lung
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Holzinger A., Dingle S., Bejarano P.A., Miller M.A., Weaver T.E., DiLauro R., Whitsett J.A. (1996) Monoclonal antibody to thyroid transcription factor-1: production, characterization, and usefulness in tumor diagnosis. Hybridoma 15(1); 49-53. 2. Bejarano P.A., Baughman R.P., Biddinger P.W., Miller M.A., Fenoglio-Preiser C., al-Kafaji B., Di Lauro R., Whitsett J.A. (1996)Surfactant proteins and thyroid transcription factor-1 in pulmonary and breast carcinomas. Modern Pathology 9(4); 445-452. 3. Bohinski. R.J., Bejarano P.A., Balko G., Warnick R.E., and Whitsett J.A. (1998) Determination of lung as the primary site of cerebral metastatic adenocarcinomas using monoclonal antibody to thyroid transcription factor-1. J. Neurooncol. 40(3); 227-231. 4. Ordonez N.G. (2000) Thyroid transcription factor-1 is a marker of lung and thyroid carcinomas. Adv. Anat. Pathol. 7(2);123-127. 5. Ikeda K., Clark J.C., Shaw-White J.R. Stahlman M.T., Boutell C.J., and Whitsett J.A. (1995) Gene structure and expression of human thyroid transcription factor-1 in respiratory epithelial cells. J. Biol. Chem. 270(14; 8108-8114
Database Name: UniProt
Accession number: P23441 (NKX21_RAT)
Species Accession: Rat
TTF-1 (Thyroid Transcription Factor 1), Homeobox protein Nkx-2.1