TSH ReceptorCatalogue number: TSHR01
The antibody against TSH receptor can be an important aid in research of thyroid diseases. In Graves' Disease (Morbus Basedow) the TSH-R expression is elevated while the detection is difficult in papillary, anaplastic and medullary thyroid carcinoma. TSH receptors are localized on the basal membrane of thyroid cells. They are integral constituents of the regulatory feedback systems coordinating the activities of the hypothalamus, pituitary and thyroid glands. In autoimmune diseases TSH-R are frequently target antigens of the immuno reaction. TSH-R autoantibodies can cause hyper-thyroidism (e.g. in Graves' disease). Human thyrotrophin receptor (aa sequence 211-414).
Immunogen: Human thyrotrophin receptor (TSH-R) (extracellular domain)
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**.
Concentration: 50 µg/ml
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Human
Incubation Time: 60 min at RT
Working Concentration: (liquid conc.) 1:10 - 1:50
Pre-Treatment: Pre-treatment with proteases not required. Antigen-unmasking with HIER may improve the staining results. If peroxidase staining systems are used, careful blocking of endogenous peroxidase should be carried out. Thyroid cells and colloid contain large amounts of oxido-reductases.
Positive Control: Normal thyroid gland
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Mizukami Y., Hashimoto T., Nonomura A., et al. (1994) Immunohistochemical demonstration of thyrotopin (TSH)-receptor in normal and diseased human thyroid tissues using monoclonal antibody against recombinant human TSH-receptor protein. J. Clin. Endocrinol. Metab. 79(2); 616-619. 2. Führer D., Holzapfel H-P., Wonerow P., Scherbaum W.A., and Paschke R. (1997) Screening for mutations in the thyrotropin receptor and gsp genes in toxic thyroidal nodules. Exp. Clin. Endocrinol. Diabetes 105 Suppl. 1; Abstract v011 p. 4. 3. Suh, J. et al. (2003) Regulation of the phosphatidylinositol 3-Kinase, Akt/protein kinase B, FRAP/mammalian target of rapamycin, and ribosomal S6 kinase 1 signaling pathways by thyroid-stimulating hormone (TSH) and stimulating type TSH receptor antibodies in the thyroid gland. J. Biol. Chem. 278:21960-21971. http://www.ncbi.nlm.nih.gov/pubmed/12668683