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Thyroglobulin

Catalogue number: TH100

CloneRBU/01
IsotypeIgG1
Units1 ml
HostMouse
Application IHC(P)

Background
In neoblastic tissue the antibody may help to identify tissues originating from thyroid gland. This is of special importance in distant metastases such as in the lung, brain, skeleton and lymph nodes. Thyroglobulin is the storage protein of the thyroid glands. The thyroid hormones are synthesized by iodination of tyrosine residues of the thyroglobulin molecule. Thyroglobulin is detected as well in the colloid of the thyroid follicles as in the glandular epithelium (granular form visible at high magnification (EM). Human Thyroglobulin, native and reduced form.

Source

Immunogen: Purified human Thyroglobulin

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Human, cross-reacting with cattle, goat, guinea pig, pig, sheep

Applications
IHC(P)

Incubation Time: 60 min at RT

Working Concentration: (liquid conc.) 1:10 - 1:50

Pre-Treatment: Protease pre-treatment is not required. However high temperature unmasking with Unmasking Fluid G (Art. No. DE007) may enhance staining. If peroxidase staining systems are used it should be taken into consideration that thyroid epithelia and colloid contain plenty of endogenous peroxidases.

Positive Control: Thyroid gland

Storage
2-8°C

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

References
1. Bellet D., Schlumberger M., Bidart J.M., Assicot M., Caillou B., Motte P. et al. (1983) Production and in vitro utilization of monoclonal antibodies to human thyroglobulin. J. Clin. Endocrinol. Metab. 56; 530-533. 2. Ormanns W. and Schäffer R. (1983) The use of monoclonal antibodies against thyroglobulin in immunoperoxidase techniques. Histochemistry 78; 177-180. 3. Wilson N.W., Pambakian H., Richardson T.C., Stokoe M.R., Makin C.A., and Heydeman E. (1986) Epithelial markers in thyroid carcinoma: an immunoperoxidase study. Histopathology 10; 815-829. 4. De Micco C., Ruf J., Carayon P., Chrestian M.-A., Henry J.-F., and Toga M. (1987) Immunohistochemical study of thyroglobulin in thyroid carcinomas with monoclonal antibodies. Cancer 59; 471-476.


Thyroglobulin

$291.00

SKU: TH100 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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