ThyroglobulinCatalogue number: TH500
In neoplastic tissue the antibody may help to identify tissues originating from thyroid gland. This is of special importance in distant metastases such as in the lung, brain, skeleton and lymph nodes. Thyroglobulin is the storage protein of the thyroid glands. The thyroid hormones are synthesized by iodination of tyrosine residues of the thyroglobulin molecule. Thyroglobulin is detected as well in the colloid of the thyroid follicles as in the glandular epithelium (granular form visible at high magnification (EM). Human Thyroglobulin, native and reduced form.
Immunogen: Purified human Thyroglobulin
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Human, crossreacting with cattle, goat, guinea pig, pig, sheep
Incubation Time: 60 min at RT
Working Concentration: (RTU) neat
Pre-Treatment: Protease pre-treatment is not required. However high temperature unmasking with Unmasking Fluid G (Art. No. DE007) may enhance staining. If peroxidase staining systems are used it should be taken into consideration that thyroid epithelia and colloid contain plenty of endogenous peroxidases.
Positive Control: Thyroid gland
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Bellet D., Schlumberger M., Bidart J.M., Assicot M., Caillou B., Motte P. et al. (1983) Production and in vitro utilization of monoclonal antibodies to human thyroglobulin. J. Clin. Endocrinol. Metab. 56; 530-533. 2. Ormanns W. and Schäffer R. (1983) The use of monoclonal antibodies against thyroglobulin in immunoperoxidase techniques. Histochemistry 78; 177-180. 3. Wilson N.W., Pambakian H., Richardson T.C., Stokoe M.R., Makin C.A., and Heydeman E. (1986) Epithelial markers in thyroid carcinoma: an immunoperoxidase study. Histopathology 10; 815-829. 4. De Micco C., Ruf J., Carayon P., Chrestian M.-A., Henry J.-F., and Toga M. (1987) Immunohistochemical study of thyroglobulin in thyroid carcinomas with monoclonal antibodies. Cancer 59; 471-476.