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Mouse anti Human T-Complex Protein 1, subunit

Catalogue number: X2069M

TCP-1b; Chaperonin containing TCP1, subunit 2 (beta); CCT-beta; CCTB

CloneF39 P7 F11
Product Type Monoclonal Antibody
Units200 µg
Species reactivity Frog
Application Immunohistochemistry
Western Blot
Product Form

The protein TCP-1 (t-complex polypeptide 1) is a subunit of the hetero-oligomeric complex CCT (chaperonin containing TCP-1) present in the eukaryotic cytosol. The CCT of eukaryotic cytosol is composed of eight different subunit species that are proposed to have independent functions in folding its in vivo substrates, the Actins and Tubulins. TCP-1 was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Sequences homologous to TCP-1 have been isolated in several other species, and the yeast TCP-1 has been shown to encode a molecular chaperone for Actin and Tubulin. TCP-1 found in mammalian cells and yeast plays an important role in the folding of cytosolic proteins.

Positive Control
Colorectal cancer tissue, Calu6 and HT29 cell lysates

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminal of the TCP-1 protein and mouse myeloma Ag8563 cells. Sequence common in human, frog, mouse, rat

Purification Method
Protein A/G Chromatography

See vial for concentration

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis
Western Blotting

Antibody can be used for Western blotting (1-2 µg/ml) and immunohistochemistry on formlin-fixed, paraffin-embedded tissue sections (1-5µg/ml). Optimal concentration should be evaluated by serial dilutions.

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

1. Kubota, H., et al. (1999). Structures and co-regulated expression of the genes encoding mouse cytosolic chaperonin CCT subunits. Eur. J. Biochem. 262(2):492-500 2. Joly, E.C., et al. (1994). cDNA encoding a novel TCP1-related protein. Biochm. Biophys. Acta. 1217(2):224-226

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide

Mouse anti Human T-Complex Protein 1, subunit


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