Survivin (IAP)Catalogue number: SUR002-G
The antibody is well suited for the immunohistochemical detection of Survivin in formaldehyde-fixed tissue slices. Survivin, an inhibitor of apoptosis protein (IAP), is expressed in the G2/M phase of the cell cycle. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increases caspase3 activity, a mechanism involved in cell death, during mitosis. The overexpression of survivin in cancer may overcome this apoptosis checkpoint and favour aberrant progression of transformed cells through mitosis. Human Survivin.
Immunogen: Synthetic C-terminal peptide sequence of human Survivin
Affinity purified IgG from rabbit serum in PBS, BSA, NaN3
Purification Method: Affinity purified IgG from rabbit serum in PBS, BSA, NaN3
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005).
Species Reactivity: Human, mouse, others not tested
Incubation Time: 60 min at RT
Working Concentration: (RTU) neat
Pre-Treatment: After formaldehyde fixation antigen unmasking at 96-100°C is recommended (use citrate buffer Art. No. DE000 or Unmasking Fluid G on glycol basis Art. No. DE007 respectively).
Positive Control: Pancreas (alpha-cells), Prostate carcinoma
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med. 1997 Aug;3(8):917-21. 2. Liggins C et al. (2003) Pediatric Dev. Pathol. 6: 392-397. 3. Kuar P., et al. (2004) Arch. Pathol. Lab. Med. 128: 39-43.
Database Name: UniProt
Accession number: O15392