Catalogue

Background
Human Ssu72 is a highly conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/termination machinery. The human Ssu72 is located on Chromosome 1p36 and seems to represent a single gene. It was identified as a pRb binding factor and was also shown to interact with TFIIB and yeast PTA1. Rabbit polyclonal antibodies were raised against SSU72 fusion protein. The ABs recognize a single band of ~30 Kda on immunoblots of various human cell lines but do not work well on mouse tissues.

Synonyms: Human Ssu72

Source

Immunogen: Full length recombinant human Ssu72 protein

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blot (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: MCF-7 and Cos-7 cells

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. St-Pierre, B., et al. 'Conserved and specific functions of mammalian ssu72' Nucleic Acids Res. 2005, 33, 464-477 2. Krishnamurthy, S., et al. 'Ssu72 is an RNA polymerase II CTD phosphatase' Mol. Cell 2004, 14, 387-394 3. Steinmetz, EJ., Brow, DA. 'Ssu72 protein mediates both poly(A)-coupled and poly(A)-independent termination of RNA polymerase II transcription' Mol Cell Biol 2003, 23, 6339-6349

Protein Reference(s)

Database Name: SwissProt

Accession Number: Q15526

Species Accession: Human

Safety Datasheet(s) for this product: