Catalogue

Background
Sphingosine-1-phosphate lyase cleaves phosphorylated sphingoid bases, such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. The catalytic activity of sphingosine 1-phosphate lyase converts sphingosine 1-phosphate to phosphoethanolamine and palmitaldehyde using Pyridoxal phosphate as a co-factor. Sphingosine 1-phosphate lyase is a type III membrane protein found in the endoplasmic reticulum. Sphingosine 1-phosphate lyase is found primarily in the liver and kidney. Sphingosine 1-phosphate lyase belongs to the group II decarboxylase family.

Synonyms: EC 4.1.2.27, SP-lyase, hSPL or Sphingosine-1-phosphate aldolase. Gene name: SGPL1 or KIAA1252

Source

Immunogen: Synthetic peptide derived from the sphingosine 1-phosphate lyase 1 protein.

Product

Product Form: Unconjugated

Formulation: Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: Lot specitic, see vial label

Applications
Antibody can be used for Western blotting (1:400 dilution). Other applications not yet tested. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Madin-Darby Bovine Kidney (MDBK) cell lysate (Cat. No. X1007)

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Van Veldhoven P.P., et al; Human sphingosine-1-phosphate lyase: cDNA cloning, functional expression studies and mapping to chromosome 10q22. Biochim. Biophys. Acta 1487:128-134(2000). 2. Reiss U., et al; Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis. J. Biol. Chem. 279:1281-1290(2004). 3. Nagase T., et al; Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.; DNA Res. 6:337-345(1999). 4. Strausberg R.L., et al; Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).

Protein Reference(s)

Database Name: SwissProt

Accession Number: O95470

Species Accession: Human

Safety Datasheet(s) for this product: