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Mouse anti Human alpha SNAP


Catalogue number: X1025

Synonyms
alpha/beta SNAP, mouse anti alpha soluble NSF attachment protein,SNAPA

Clone4E4
IsotypeIgG1
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Species reactivity Bovine
Human
Rat
Application Immunoprecipitation
Western Blot
Product Form
Unconjugated

Background
SNAPs (soluble NSF attachment proteins), acting in concert SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (or bSNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas a-SNAP is expressed in all mammalian tissues, b-SNAP is expressed only in brain.

Positive Control
Rat Brain Lysate

Immunogen
Recombinant human a-SNAP protein

Concentration
See vial for concentration

Formulation
Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5

Functional Analysis
Western Blotting

Source
Hybridoma 4E4 produced by the fusion of splenocytes from mice immunized with recombinant human a-SNAP protein and mouse myeloma cells.

Product
Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5

Applications
Antibody can be used for Western blotting (1:5000 dilution) and immunoprecipitation on native and denatured samples. Optimal concentration should be evaluated by serial dilutions.

Specificity
Human alpha SNAP

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Reagents are stable for the period shown on the vial label when stored properly

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Mouse anti Human alpha SNAP

$254.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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