Catalogue

Background
Atrazine is a herbicide against pre- and post-emergence broadleaf and grassy weeds. It is a very persistent compound and it has been banned in the EU since 2004. Its main toxic effect is as endocrine diruptor.

Source
Serum of sheep immunised with BSA-atrazine conjugate, and purified using caprylic acid

Product
Each vial contains 0.5 ml of purified (caprylic acid) sheep polyclonal IgG containing 0.02% sodium azide.

Specificity
Immunogen: BSA-atrazine conjugate, Target: Atrazine, CAS no.: 1912-24-9. The specificity of the antibody was determined by measuring the cross-reactivity with a range of compounds in ELISA.

Species Reactivity: Cross Reactivity: Atrazin 100% Propazine 15% Simazine 4% Ametryn 2500% Prometryn 1700% Simetryn 280% Terbutylazine 2% Atrazine-desethyl 7% Atrazine desisopropyl 1% Atrazine-2-hydroxy 1% Cinosulfuron <0.1% Prosulferon <0.1% Triasulfuron <0.1% Chlorpyrifos <0.1%Clofibrinezuur <0.1% Deltametrin <0.1% Diclofenac 1% Etrimfos 1% Erythromycine <0.1% Fenitrothion <0.1% Fenofibraat <0.1% Atrazine 21% Ibuprofen <0.1% Malathion <0.1% Maleaminezuur <0.1% Metoprolol <0.1% Metacrifos <0.1% 4-n-Nonylfenol 2% Permetrin <0.1% Pirimifos-ethyl <0.1% Sulfadimidine <0.1% Vinclozolin <0.1%

Applications
Depending on conjugate: dilution 1/500 . (The titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Suggested concentration to use in ELISA: 1/500 from the delivered solution. Plates are coated with 400 ng/ml OVA-conjugated Atrazine. HRP-conjugated anti-sheep IgG as a tracer 1/8,000

Storage
Store at 4 °C or aliquot and store at -20 °C for longer periods. Precautions should be taken upon storage for longer periods. Problems of long term stability may occur with highly diluted solutions. For lon term storage purposes in solution the addition of sodium azide at 0.02 % is advised with the appropriate precautions of use.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.