Serotonin (5-HT)Catalogue number: SE100
The polyclonal antibody to serotonin is suitable for immunohistochemistry. It labels serotonin as well in normal cells and tissues as in carcinoids. Serotonin is a biogenic amine (5-Hydroxy-Tryptamine) which is produced in many eucaryontic cells. In Vertebrates it plays an important role as a neurotransmitter substance of the central and the peripheral nervous system. In the stomach and intestine it is produced by s.c. enterochromaffine cells in epithelia cell layers. In the blood it is mainly found in thrombocytes, basophilic granulocytes and in the granula of mast cells. Serotonin (5-Hydroxy-tryptamine).
Immunogen: Hapten conjugated synthetic Serotonin (5-Hydroxy-tryptamine)
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005).
Species Reactivity: Human and other spec.
Incubation Time: 60 min at RT
Working Concentration: (liquid conc.) 1:10 - 1:50
Pre-Treatment: Pre-treatment with proteases is not required. Unmasking with unmasking fluid G (Code No. DE007) may be advantages.
Positive Control: Gastric and intestinal epithelia
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!