Catalogue

Background
The S-100 antibody has frequently been used for the detection of melanoic tumours. In brain tumours and metastases the antibody may be used for differentiation between glia derived tissue and tissues derived from other cell types. S-100 helps in differentiation of Melanoma and Schwannoma from other soft tissue tumours. It is also found in tumours of the salivary glands. S-100 is a homo- or heterodimer filament protein, composed of an alpha and a beta chain. In normal tissues it is found in glia and ependyma of the brain. In the periphery it is found in Schwann Cells, Melanocytes and in Langerhans Cells of the skin. In lymph nodes Interdigitating Cells are labelled. S-100 A0, which is exprimed e.g. by neurons, heart muscle and monocytes, will not be detected by this monoclonal antibody. S-100 ß, S-100 A (alpha/beta) and S-100 B (beta/beta), not reactive with S-100 A0 (alpha/alpha), Calmodulin, Parvalbumin and other Ca-binding proteins.

Source

Immunogen: Purified S-100ß protein from bovine brain

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 200 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 200 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Cattle, human, cat, rabbit, rat, pig

Applications
IHC(P)

Incubation Time: 60 min at RT

Working Concentration: (liquid conc.) Dilution 1:20 - 1:50 IHC(P)

Pre-Treatment: Formaldehyde-fixed tissue should be pre-treated with 0.1% pronase (Art. No. DE110) to give best results. Unmasking methods involving microwave or steam cooking are not recommended!

Positive Control: Skin, myelinated nerves

Storage
2-8°C

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Moore B.W. (1965) A soluble protein characteristic of the nervous system. Biochem. Biophys. Res. Comm.; 19; 739-744. 2. Kindblom L.-G, Lodding P., Rosengren L., Baudier J., Haglid K. (1984) S-100 protein in melanocytic tumors. Acta path. microbiol. Immunol. Scand. Sect. A 92; 219-230. 3. Lauriola L., Michetti F., Sentinelli S., Cocchia D. (1984) Detection of S-100 labelled cells in nasopharyngeal carcinoma. J. Clin. Pathol. 37; 1235-1238. 4. Winek R.R. Scheithauer B.W. Wick M.R. (1989) Meningioma, meningeal hemangiopericytoma (angioblastic meningioma), peripheral hemangiopericytoma, and acoustic schwannoma. A comparative immunohistochemical study. Am. J. Surg. Pathol. 13(4); 251-261.

Safety Datasheet(s) for this product: