Catalogue

Background
The reactivity of the antiserum is restricted to platelet factor 4 as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified immunogen. In precipitating techniques as electroimmunodiffusion (Laurell), immunoelectrophoresis and single and double radial immunodiffusion to identify the presence platelet factor 4. This antiserum has not been tested in more sensitive assays as nephelometry, ELISA or use in immunohistology, but this does not exclude such use if proper controls are included. Measurement of platelet factor 4 is of significance for diagnosis of platelet disorders involving platelet alpha granules and to demonstrate increased platelet secretion in vivo. Reduced levels are measured in gray platelet syndrome, whereas elevated levels have been reported in cases as coronary artery disease, myocardial infarction, disseminated intravascular coagulation, cerebrovascular disease and cancer.

Source
Platelet factor 4 is a low molecular weight (MW 7,800) heat stable protein, secreted by alpha granules of the platelets. Purified platelet factor 4 used for immunization of the rabbits was isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Product
Delipidated, heat inactivated, lyophilized, stable whole antiserum Sodium azide. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.

Formulation: Delipidated, heat inactivated, lyophilized, stable whole antiserum Sodium azide. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.

Specificity
Precipitating polyclonal Rabbit antiSerum to Human platelet factor 4.

Species Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail. In double radial immunodiffusion a reaction has been observed with Bovine, Cat, Dog and Monkey.

Applications
Precipitation assays. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In electroimmunodiffusion the amount of antiserum required in the agarose gel is usually between 1 and 2%, depending on the test arrangement. The amount of platelet factor 4 precipitated by 1 ml antiserum is approximately 0.1 mg.

Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

Protein Reference(s)

Database Name: UniProt

Accession Number: P02776

Safety Datasheet(s) for this product: