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Protein A/G Agarose


Catalogue number: X1202

Synonyms
Protein A/G Agarose

Product Type Antibody Purification Reagent
Units25 ml
Application Antibody Purification
Product Form
Agarose

Background
Our recombinant Protein A and G combines the binding characteristics of Protein A and Protein G, providing an easy way to purify most immunoglobulins. Recombinant Protein A/G Agarose is produced by mixing equal amounts of our superior recombinant Protein A and Protein G agarose products.

Concentration
See vial for concentration

Formulation
Provided as a 50% aqueous suspension of Protein A/G in 20% ethanol solution.

Applications
Ligand Density: approximately 6 mg/ml of Protein A and 2 mg/ml of Protein G of drained gel Dynamic Binding Capacity: approximately 18 to 35 mg human IgG per ml of drained gel (estimated in a 0.7x5cm column at a linear flow rate of 300 cm/h under the following conditions: binding buffer: 0.1 M PBS pH 7.0, Eluting buffer 0.1 M citrate pH 3.0. 2.8 times excess IgG of the binding capacity was applied. Bead Structure: 4% highly cross-linked agarose Bead Size Range: 45 to 165 µm Mean Bead Size: approximately 90 µm Maximum Linear Flow Rate: >1300 cm/hour at 25 deg C in an XK 16/20 column, bed height 5 cm pH Stability: Long term storage - 3 to 9; short term storage - 2 to 10 Chemical Stability: IgG binding capacity and recovery maintained after storage for (a) 7 days at 40º C in 3M NaSCN, 6M guanidine-HCl, 0.1M glycine (pH3.0), 70% ethanol; 10 hours at 23 º C in 8M urea, 0.1M glycine (pH 11), 5.8M acetic acid (pH 2.0) Physical stability: Negligible volume variation due to changes in pH or ionic strength Sanitization: Sanitize the packed column with 2% Hibitane/20% ethanol or with 70% ethanol

Storage
Product should be stored at 4-8ºC. DO NOT FREEZE

Ship Conditions
Ship at ambient temperature, do not freeze, refrigerate upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Protein A/G Agarose

$795.00