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p16 INK4a (DCS-50)


Catalogue number: P16002-L

CloneDCS-50
IsotypeIgG1
Product Type Monoclonal Antibody
Primary Antibodies
Units1ml
HostMouse
Species reactivity Human
Application Immunohistochemistry (Frozen & Paraffin Sections)
Immunoprecipitation
Western Blot
Background
The antibody is suitable to detect p16 in different tissues. Staining results may be cytoplasmatic or nuclear. Nuclear staining should be mor specific.

Human p16 INK4a (syn. CDKN2; MTS-1) protein is an inhibitor of Cyclin-Dependent-Kinases 4 and 6 (cdk4/cdk6). CDK4 is an important enzyme for the progression of the cell cycle during the G1-Phase. By it's inhibitory action p16 is an important regulator in the cell cycle. The gen encoding for p16 is deleted in many tumour cell lines. In adenocarcinoma of the cervix uteri usually p16 levels are increased. Endocervical p16-overexpression is mostly associated with "high risk" HPV-Typing. In other tumour types as squamous epithelial carcinoma in cervix uteri and head and neck carcinoma p16 expression usually is reduced.

Positive Control
Colon carcinoma, HPV-pos. cervical carcinoma

Pre-Treatment
Pre-treatment with Unmasking Fluid C (Citrate Buffer, Art. No. DE000) or Unmasking Fluid G (Art. No. DE007) at 96-100 degree of Celsius is recommended for paraffin sections.

Immunogen
Recombinant human p16 INK4a molecule

Purification Method
Protein A affinity purification.

Working Concentration
(lyophilisate) 1:10

Concentration
50 µg/ml after reconstitution in 1 ml distilled water

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Source
Monoclonal antibody DCS-50 is produced after immunisation of Balb/c mice with the full length recombinant p16 INK4a protein of human origin.

Product
Protein A affinity purified antibody lyophilized from PBS pH7.4 with BSA and Na-Azide 0.09%.

Applications
IHC (C,P), IP

Cross Reactivity
Human, other species not tested

Specificity
p16 INK4a

Storage
2-8°C

References
1. Koh J., Enders G.H., Dynlacht B.D., and Harlow E. (1995) Tumour-derived p16 allels encoding proteins defective in cell cycle inhibition. Nature 375; 506-510.
2. Reed A.L., Califano J., Cairns P., Westra W.H., Jones R.M., et al. (1996) High frequency of p16 (CDKN2/MTS-1/INK4A) inactivation of head and neck squamous cell carcinoma. Cancer Res. 56(16); 3630-3633.
3. Geradts J., Hruban R.H., Schütte M., Kern S.E., and Maynard R. (2000) Immunohistochemical p16INK4a analysis of archival tumors with deletion, hypermethylation, or mutation of the CDKN2/MTS1 gene. A comparison of four commercial antibodies. Appl. Immun
4. Negri G., Egarter-Vigl E., Kasal A., Romano F., Haitel A., Mian C. (2003) p16INK4a is a useful marker for the diagnosis of adenocarcinoma of the cervix uteri and its precursors: an immunohistochemical study with immunocytochemical correlations. Am J. S
5. Kotaro R. Shibata,Tomoki Aoyama,Yasuko Shima et al. (2007) Expression of the p16INK4A Gene Is Associated Closely with Senescence of Human Mesenchymal Stem Cells and Is Potentially Silenced by DNA Methylation During In Vitro Expansion Stemm Cells vol. 9; 2371–2382.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

p16 INK4a (DCS-50)

$599.00

SKU: P16002-L Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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