Mouse IgG2a FITC Negative ControlCatalogue number: 02A2
|Product Type||Single-Color Negative Control|
Negative control for immunofluorescence staining with FITC conjugated mouse monoclonal antibodies. Assessment of non-specific binding of mouse monoclonal antibodies to human cell surface antigens.
Purified mouse IgG2a conjugated to FITC, as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood.
PBMC: Add10 μl of MAB/10^6 PBMC in 100 μl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze.
WHOLE BLOOD: Add10 μl of MAB/100 μl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Working Concentration: Titered for flow cytometry
Product should be stored at 4-8ºC. DO NOT FREEZE. Store protected from light. Reagent is stable for the period shown on the vial when stored properly.
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Room Temperature
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Accession number: Swiss Prot P01863
Reagent is stable for the period shown on the vial when stored properly.
Mouse IgG2a FITC Negative Control