Mouse Ig and IgG subclasses standard SerumCatalogue number: NOR-02
This standard is prepared by pooling serum obtained from a large number mice belonging to a-allotype and b-allotype stains with the following assigned values: IgG1--2.00 mg/ml. IgG2a--8.54 mg/ml. IgG2b--1.25 mg/ml. IgG3--0.754 mg/ml. IgA--2.75 mg/m. IgM--0.277 mg/ml. The determination of the concentration of each of the four subclasses was carried out by single radial immunodiffusion (RID, Mancini) in agar gel Nordic Nr. 2 using subclass-specific polyclonal antisera and purified mouse immunoglobulins as primary standards. Allotypic differences in mouse IgG2a may interfere with the determination of this subclass. At least eight allelic variants of IgG2a have been identified. Most laboratory mouse strains fall into one of these allogeneic groups. Mouse Standard Serum NOR-02 contains IgG2a of both allelic types Igh-1aand Igh1b and cab be used for the quantitative determination of IgG2a in the majority of mouse strains, including Balb/C, C3H, CBA/J, SJL/J and SM/J. Mouse standard serum NOR-02 is intended for use as a calibrator to prepare standard curves in quantitative determinations of the immunoglobulins IgA and IgM and IgG subclasses.
Delipidated, lyophilized serum. No preservative added
Mouse standard Serum with assigned values for IgA, IgM and IgG subclasses.
The product is shipped lyophilized at ambient temperature. The lyophilized content is reconstituted by adding 0.5 ml sterile water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted serum may be divided into small aliquots, frozen at or below -20°C.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Mouse Ig and IgG subclasses standard Serum