Mouse anti Goat / Sheep IgG, conjugated with HRPCatalogue number: X1206M
|Product Type||Second Step Reagents|
Monoclonal Anti-Goat/Sheep IgG - Peroxidase Conjugate is is derived from the GT-34 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with goat IgG conjugated to purified horseradish peroxidase. Purified mouse immunoglobulin is conjugated to peroxidase by protein cross-linking with 0.2% glutaraldehyde. The conjugated antibody is lyophilized from M phosphate buffered saline containing 0.01 1% BSA and 0.01% thimerosal as preservative.Peroxidase Monoclonal Anti-Goat/Sheep IgG recognizes an epitope located on the heavy chain of both goat IgG1 and IgG2. The product shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components, or with IgG derived from the following species: human, guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat.
Synonyms: Mouse anti-sheep and goat-HRP
Product Form: HRP conjugate
Formulation: Provided as solution in phosphate buffered saline, pH 7.3, with 1% BSA and 0.1% 2-chloroacetamide
Concentration: See vial for concentration
Antibody can be used for the localization of goat or sheep IgG by ELISA, immunobotting and immunohistochemistry. Preparation Note: Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Functional Analysis: Western Blotting
Positive Control: Specificity: The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Ship Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
1. Artman, M., et al., K ATP channels of primary human coronary artery endothelial cells consist of a heteromultimeric complex of Kir6.1, Kir6.2, and SUR2B subunits. J. Mol. Cell. Cardiol. 37, 857-869, (2004) Abstract 2. Vicente, C.P., et al., Heparin cofactor II inhibits arterial thrombosis after endothelial injury J. Clin. Invest. 109, 213-219, (2002) Abstract 3. Bír, A., et al., Studies on the interactions between C-reactive protein and complement proteins Immunology 121, 40-50, (2007) Abstract 4. van Beekum, O., et al., The Adipogenic Acetyltransferase Tip60 Targets Activation Function 1 Of Peroxisome Proliferator-activated Receptor Gamma. Endocrinology 149, 1840-9, (2008) Abstract 5. Oksala, N., et al., ADAM-9, ADAM-15, And ADAM-17 Are Upregulated In Macrophages In Advanced Human Atherosclerotic Plaques In Aorta And Carotid And Femoral Arteries—Tampere Ann. Med. 41, 279-90, (2009) Abstract
Mouse anti Goat / Sheep IgG, conjugated with HRP