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Mouse anti Goat / Sheep IgG, conjugated with HRP


Catalogue number: X1206M

Synonyms
Mouse anti-sheep and goat-HRP

CloneGT-34
IsotypeIgG1
Product Type Second Step Reagents
Units500 µl
HostMouse
Species reactivity Bovine
Goat
Sheep
Application ELISA
Western Blot
Product Form
HRP conjugate

Background
Monoclonal Anti-Goat/Sheep IgG - Peroxidase Conjugate is is derived from the GT-34 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with goat IgG conjugated to purified horseradish peroxidase. Purified mouse immunoglobulin is conjugated to peroxidase by protein cross-linking with 0.2% glutaraldehyde. The conjugated antibody is lyophilized from M phosphate buffered saline containing 0.01 1% BSA and 0.01% thimerosal as preservative. Peroxidase Monoclonal Anti-Goat/Sheep IgG recognizes an epitope located on the heavy chain of both goat IgG1 and IgG2. The product shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components, or with IgG derived from the following species: human, guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat.

Positive Control
Specificity: The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.

Concentration
See vial for concentration

Formulation
Provided as solution in phosphate buffered saline, pH 7.3, with 1% BSA and 0.1% 2-chloroacetamide

Functional Analysis
Western Blotting

Applications
Antibody can be used for the localization of goat or sheep IgG by ELISA, immunobotting and immunohistochemistry. Preparation Note: Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Artman, M., et al., K ATP channels of primary human coronary artery endothelial cells consist of a heteromultimeric complex of Kir6.1, Kir6.2, and SUR2B subunits. J. Mol. Cell. Cardiol. 37, 857-869, (2004) Abstract 2. Vicente, C.P., et al., Heparin cofactor II inhibits arterial thrombosis after endothelial injury J. Clin. Invest. 109, 213-219, (2002) Abstract 3. Bír, A., et al., Studies on the interactions between C-reactive protein and complement proteins Immunology 121, 40-50, (2007) Abstract 4. van Beekum, O., et al., The Adipogenic Acetyltransferase Tip60 Targets Activation Function 1 Of Peroxisome Proliferator-activated Receptor Gamma. Endocrinology 149, 1840-9, (2008) Abstract 5. Oksala, N., et al., ADAM-9, ADAM-15, And ADAM-17 Are Upregulated In Macrophages In Advanced Human Atherosclerotic Plaques In Aorta And Carotid And Femoral Arteries—Tampere Ann. Med. 41, 279-90, (2009) Abstract

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Mouse anti Goat / Sheep IgG, conjugated with HRP

$113.00

SKU: X1206M Categories: ,