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Mouse anti Human CD56

Catalogue number: 561

CloneC5.9
IsotypeIgG2b
Product Type Monoclonal Antibody
Units250 µg
HostMouse
Species reactivity Human
Application Flow Cytometry
Immunocytochemistry
Immunohistochemistry (paraffin)

Background
CD56, M.W. 150,000, is expressed on approximately 10-25% of human peripheral blood lymphocytes. CD56 (NKH-1) is expressed on human peripheral blood natural killer cells, representing a pan NK-cell antigen. Expressed on non-MHC-restricted cytotoxic T cells. ExalphaÕs CD56, clone C5.9 has been recognized as the superior CD56 antibody for Flow Cytometry applications because of itÕs specific and intense staining. In fact, our CD56 is bright enough to be used in FITC! Now this antibody is proven to work in formalin fixed paraffin embedded section. Available concentrated, pre-diluted in dropper bottles, or conjugated to several flurochromes for manual use or automated applications.

Synonyms: CD56, NCAM, Neural Cell Adhesion Molecule

Source

Immunogen: CD56=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with KG1a cell line.

Product

Product Form: Unconjugated

Formulation: Filtered includes 0.08% azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Antibody can be used for Immunohistochemistry (2-5 µg/ml, formalin-fixed, paraffin embedded tissues). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Flow Cytometry

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Reagents are stable for the period shown on the vial label when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Hercend T., Griffin J.D., Benussan A., Schmidt R.E., Edson M.A., Brennen A., Murray C., Daley J.F., Schlossman S.F., and Ritz J. Generation of Monoclonal Antibodies to a Human Natural Killer Clone. Characterization of Two Natural Killer-Associated Antigens, NKH1 and NKH2, Expressed on Subset of Large Granular Lymphocytes. J. Clin. Invest. 1985,75: 932.

2. Lanier, L.L., Le, A.M., Civin, C.I., Loken, M.R., and Phillips, J.H. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) Antigen Expression on Human Peripheral Blood NK Cells and Cytotoxic T Lymphocytes. J Immunol. 1986, 136: 4480.

3. Schmidt RE, Murray C, Daley JF, Schlossman SF, and Ritz J, J. A subset of natural killer cells in peripheral blood displays a mature T cell phenotype. J Exp Med 1986, 164: 351-356

4. Caligiuri M, Murray C, Buchwald D, Levine H, Cheney P, Peterson D, Komaroff AL, and Ritz J. Phenotypic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome. Immunol 1987, 139: 3306-3319

5. Hercend T. and Schmidt R.E. Characteristics and uses of natural killer cells. Immunol Today 1988, 9: 291-293.

6. Muench, Marcus O., et al. Isolation of Definitive Zone and Chromaffin Cells Based upon Expression of CD56 (Neural Cell Adhesion Molecule) in the Human Fetal Adrenal Gland, J Clinical Endocrinology & Metabolism. 2003, 88: 3921-3930,

PRODUCT SPECIFIC REFERENCES
1. Horikawa, M., et al. Abnormal Natural Killer Cell Function in Systemic Sclerosis: Altered Cytokine Production and Defective Killing Activity. Journal of Investigative Dermatology 2005, 125: 731-737

2. Ishimoto, H., et al. Midkine, a heparin-binding growth factor, selectively stimulates proliferation of definitive zone cells of the human fetal adrenal gland. Journal of Endocrinol. Metab 2006, 91: 4050-4056

3. Muench, M., et al. Isolation of Definitive Zone and Chromaffin Cells Based upon Expression of CD56 (Neural Cell Adhesion Molecule) in the Human Fetal Adrenal Gland. Journal of Clinical Endocrinol. Metab 2003, 88: 3921-3930

4. Miles, L.A., et al. Cell-Surface Actin Binds Plasminogen and Modulates Neurotransmitter Release from Catecholaminergic Cells. Journal of Neuroscience 2006, 26: 13017-13024

Protein Reference(s)

Database Name: UniProt

Accession number: P13591


Mouse anti Human CD56

$313.00

SKU: 561 Categories: ,