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Laminin, human


Catalogue number: LA24811

Units0.5 ml
HostRabbit
Application ELISA
IFA
IHC(P)
RIA
Background
The antibody against Laminin primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion. Laminin is also found in extracellular matrix not related to basal membranes, e.g. in the proximity of neurons especially during development, regeneration and tumour development. Laminins are glycoproteins of the extracellular matrix, especially of the basal membranes. They are multi-functional molecules which play an important role during development and differentiation of organs and for cell movements. These properties rely on the interaction of laminins with receptors on cell surfaces like integrins, collagen IV, proteoglycans. Laminins consist of 2 heavy chains (A and M, 300-400 kDa) and 1 light chain (B1, B2 or S, 200 - 220 kDa). Human laminin fragment P1 100%, human collagen type I and IV <0.1%, human fibronectin <0.1% (RIA at 1:1000 dilution).

Positive Control
Human skin

Pre-Treatment
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Immunogen
Purified laminin P1 fragment from human placenta

Purification Method
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

Working Concentration
(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:250, ELISA ? 1:200 (OD ? 500)

Concentration
app. 1 mg/ml

Incubation Time
IHC(P) 60 min at RT or 2-8°C over night

Secondary Reagents
Anti-rabbit IgG-conjugates

Product
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

Applications
IFA, IHC(P), ELISA, RIA

Cross Reactivity
Human

Storage
-20°C

References
See list of references in BIOLOGO ECM brochure.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Safety Datasheet(s) for this product:

Laminin, human

$822.00

SKU: LA24811 Categories: ,