Mouse anti Human IgG2a / IgG2b, conjugated with Cy5RPE

Catalogue number: X1049

Synonyms
IgG2a & IgG2b Negative Control

Product Type	Single-Color Negative Control
Units	100 Tests
Host	Mouse
Species reactivity	Human
Application	Flow Cytometry

Product Form
RPE-Cy-5

Background
Negative control for Immunofluorescence staining with Pure, FITC, Biotin and PE of mouse monoclonal antibodies. Assessment of non-specific binding of mouse monoclonal antibodies to human cell surface antigens.

Purification Method
Protein A/G Chromatography

Concentration
Titered for flow cytometry

Formulation
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Functional Analysis
Flow Cytometry Staining

Applications
Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood. PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: Excites at 488nm and emits at 670nm. Store protected from light.

Storage
Product should be stored at 4-8ºC. DO NOT FREEZE

Ship Conditions
Room Temperature

Product Stability
Reagents are stable for the period shown on the vial label when stored properly

References
Cy-5 Portions of this product is manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.