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Ig Kappa Chain, human


Catalogue number: IG510

CloneKP-53
IsotypeIgG2b
Units5 ml
HostMouse
Application IHC(C
P)
Background
The anti-kappa antibody may be used for the detection of abnormal immunoglobulin production, e.g.. monoclonal gammopathy with increased production of both free and bound kappa chains. In this context the differentiation from lambda light chains or heavy chains is of importance. The antibody stains B-cells in lymphoidal tissue and peripheral blood as well as plasma cells expriming kappa chains (especially useful in formaldehyde-fixed paraffin sections). Reed-Sternberg-Cells may be stained if exogenous Ig has been absorbed. The antibody may be helpful for the detection of the monoclonal nature of lymphoidal neoplasms. Immunoglobulins are composed of heavy and light chains and the light chains may be of the kappa or lambda type. In humans app. 65% of light chains are kappa chains. In gammopathy these light chains can occur in high concentration as free kappa chains. Human kappa light chain (free and bound), no cross-reactivity with lambda-chains.

Positive Control
Tonsil

Pre-Treatment
Pre-treatment with pronase 0,1% (Art. No. DE110) or unmasking fluid G (Art. No. DE007)

Immunogen
Purified kappa light chain from Bence Jones' gammopathy

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Working Concentration
(RTU) neat

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Applications
IHC(C, P)

Cross Reactivity
Human

Storage
2-8°C

References


Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

Ig Kappa Chain, human

$158.00

SKU: IG510 Categories: ,