Mouse anti HIV reverse transcriptase (HIV-RT), conjugated with Biotin

Catalogue number: F110M

Clone	AS35
Isotype	IgG1
Product Type	Monoclonal Antibody
Units	100 µg
Host	Mouse
Application	Enzyme Immunoassay Immunoprecipitation

Product Form
Biotin

Background
Anti-Human Immunodeficiency Virus Reverse Transcriptase is an antibody meant to target the Reverse Transcriptase (RT) efficiency in HIV infections. RT allows the viral RNA to be transcribed to host DNA and synthesize new DNA composed of the virusÕ genome. Because RT also exhibits RNaseH activity, it enables the production of a DNA/RNA hybrid used for synthesis. Additionally, it can cause errors during transcription, creating difficulties in isolating the virus and promoting further infection.

Positive Control
Specificity: Recognizes an epitope on HIV-RT that is different from AS34 epitope.

Immunogen
Recombinant immuno-deficiency virus reverse transcriptase (HIV- RT).

Purification Method
Protein A/G Chromatography

Purity
≥95%

Concentration
See vial for concentration

Formulation
Provided as a 0.2µm sterile-filtered solution in Ca++ & Mg++ free Dulbecco's PBS with 0.08% sodium azide.

Functional Analysis
ELISA

Applications
Applications: ELISA: The biotin form of this antibody has been used as solution phase Ab at 2 ug/ml in a two site ELISA with AS34. Typically the ELISA has a working range of 2-500 ng/ml and a sensitivity of <1 ng/ml HIV-RT. Recommended sample volumes are 50-200 ml. Immunoprecipitation: This antibody has been used to immunoprecipitate > 90% HIV-RT in solution using a double-antibody method (Midgley and Hepburn). Immunoprecipitation has also been accomplished using Protein G agarose.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Room Temperature

Product Stability
Products are stable for one year from purchase when stored properly

References
Midgley, AR and Hepburn, MR, Methods Enzymol., 70, 266, 1980.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf