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Mouse anti HA Tag


Catalogue number: X2680M

CloneHA.C5
IsotypeIgG3
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Application Dot Blot ELISA
Immunoprecipitation
Immunostaining
IP
WB
Western Blotting
Product Form
Unconjugated

Background
An HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein when doing binding studies with transfected cells. Reacts with any protein tagged with the HA sequence.

Positive Control
Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins

Immunogen
YPYDVPDYA (HA) synthetic peptide conjugated to KLH

Purification Method
Protein A/G Chromatography

Concentration
See vial for concentration

Formulation
10mM PBS, pH 7.2, with 0.05% Sodium Azide

Functional Analysis
Western Blotting

Applications
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature). IS: 1:500-2000 dilution. Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Mouse anti HA Tag

$180.00

SKU: X2680M Categories: ,