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Mouse anti HA Tag


Catalogue number: X1000

Synonyms
Mouse Anti HA, hemaglutinin

Clone12CA5
IsotypeIgG2b
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Application Enzyme Immunoassay
IHC
Immunohistochemistry
Immunoprecipitation
IP
Western Blot
Product Form
Unconjugated

Background
An HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein when doing binding studies with transfected cells. Balb/C mice were immunized with a 9 aa petide to influenza virus hemagglutinin (HA). The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by western blot. The antibody was purified to >95% homogeneity by standard chromatographic techniques.

Positive Control
HA taged proteins

Immunogen
Balb/C mice were immunized with a 9 amino acid petide to influenza virus hemagglutinin

Purification Method
Protein A/G Chromatography

Purity
>95% by SDS Page

Concentration
See vial for concentration

Formulation
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis
Western Blotting

Product
The product contains 100 µg of purified antibody in PBS containing 0.08% sodium azide

Applications
Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Mouse anti HA Tag

$254.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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