Sheep anti Human Guanalyate Kinase (PDZ 4-5) (MAGI)

Catalogue number: K110P

Synonyms
Sheep anti-Membrane-associated guanylate cyclase of inverted orientation-3, PSD95 DiscsLarge Z01 domains 4-5

Isotype	IgG
Product Type	Polyclonal Antibody
Units	250 µg
Host	Sheep
Species reactivity	Human
Application	Western Blot

Product Form
Unconjugated

Background
Membrane-associated guanylate kinase with inverted orientation (MAGI3) is a novel, 130 kDa guanylate kinase which is part of a family of multi-PDZ domain containing guanylate kinases. These guanylate kinases are localized to epithelial cell tight junctions. MAGI3 and PTEN/MMAC cooperate to modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions the PTEN/MMAC phosphatase to specific subcellular locations that are involved with the regulation of cell proliferation and survival. MAGI3, as well as other proteins in this family, are made up of an N-terminal guanylate kinase domain, followed by a WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, which is then followed by 6 PDZ domains.

Immunogen
Recombinant protein corresponding to PDZ domains 4-5 of MAGI-3.

Purification Method
Ammonium Sulfate Precipitation

Concentration
See vial for concentration

Formulation
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis
Western Blotting

Applications
Antibody detects MAGI-3 by Western blot at 1-10 µg/ml. Detects a 130 kDa band in cells transfected with full-length MAGI-3 protein. Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Wu, Y., et al. Interaction of the tumor suppressor PTEN/MMAC with a PDZ domain of MAGI3, a novel membrane-associated guanylate kinase. J. Biol. Chem. 2000, 275, 21477-21485

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
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