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GHrelin, desacylated ELISA


Catalogue number: GH-97752

Units96 Wells
Application ELISA
Background
GHrelin, a novel growth hormone releasing peptide is an acylated peptide that stimulates the release of growth hormone from pituitary. It was isolated from rat stomach and the structure was determined as a peptide consisting of 28-amino acid by Dr.Kenji kangawa(National Cardiovascular Center in Japan).The Ser3 residue of GHrelin is modified by n-octanoic acid ,a modification necessary for hormone activity. This Desacyl-Ghreline ELISA kit measures only the Desacyl-Ghreline based on the principle of 2 Site Sandwich enzyme-linked immunosorbent assay(ELISA). It can detect not only human Desacyl-Ghreline but also rat/mouse Desacyl-Ghreline(1-28). This kit is manufactured using the high specific antibody pairs generated by Dr.kangawa and by following his protocol.(patent pending?PCT WO 01/07475 A1) This product is useful for Diabetes / Obesity study. GHrelin, Desacyl-Ghrelin, GHrelin unacylated, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, M46 protein, GHRL.

Immunogen
Desacyl-Ghrelin

Purification Method
1. Standard ( Lyophilized ) 1 vial, 2. Assay buffer 22 ml, 3. Antibody coated plate 8x12 wells, 4. HRP conjugated antibody 250 ?l, 5. HRP dilution buffer 22 ml, 6. Substrate solution 22 ml, 7. Stop reagent ( 0.5 mol/L H2SO4 ) 6 ml, 8. Washing buffer concentrate 40 ml.

Working Concentration
(Kit)

Product
1. Standard ( Lyophilized ) 1 vial, 2. Assay buffer 22 ml, 3. Antibody coated plate 8x12 wells, 4. HRP conjugated antibody 250 ?l, 5. HRP dilution buffer 22 ml, 6. Substrate solution 22 ml, 7. Stop reagent ( 0.5 mol/L H2SO4 ) 6 ml, 8. Washing buffer concentrate 40 ml.

Applications
ELISA

Cross Reactivity
Human, mouse, rat

References


Caution
*These ELISA kits are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

GHrelin, desacylated ELISA

$1,423.00

SKU: GH-97752 Categories: ,