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Mouse anti Green Fluorescent Protein (GFP)

Catalogue number: X2678M

CloneGF28R
IsotypeIgG1
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Application Dot Blot
ELISA
Immunoprecipitation
Immunostaining
Western Blotting

Background
GFP protein is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Reacts with Native and Dentured froms of GFP and its varients such as EGFP, YFP, EYFP, CFP.

Source

Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates

Product

Product Form: Unconjugated

Formulation: 10mM PBS, pH 7.2, with 0.05% Sodium Azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Ship Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.


Safety Datasheet(s) for this product:
Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Mouse anti Green Fluorescent Protein (GFP)

$235.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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