Mouse anti Green Fluorescent Protein (GFP)Catalogue number: X2678M
|Product Type||Monoclonal Antibody|
GFP protein is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Reacts with Native and Dentured froms of GFP and its varients such as EGFP, YFP, EYFP, CFP.
Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates
Product Form: Unconjugated
Formulation: 10mM PBS, pH 7.2, with 0.05% Sodium Azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Safety Datasheet(s) for this product:
Mouse anti Green Fluorescent Protein (GFP)