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E-Cadherin (Uvomorulin)


Catalogue number: CA150

CloneSPM471
IsotypeIgG2a
Units1 ml
HostMouse
Application IHC(C
P)
Background
The antibody to E-cadherin is well suited to detect this glycoprotein in different types of epithelia. The localization is usually limited to the lateral cell membrane. E-Cadherin (Uvomorulin, ARC-1) is a Ca2+-dependent trans-membrane protein, which plays an important role for the cell connection in epithelia. Ligands for E-Cadherin are catenins, which bind to actin filaments of the cyto-skeleton. The cell to cell adhesion during embryo genesis is dependent of the interrelation of these three components. Reduced expression of E-Cadherin in carcinoma has been discussed as a factor influencing tumour invasion and bad prognosis of cancer. E-Cadherin 120 kDa.

Positive Control
Small intestine, appendix, corneal epithelium

Pre-Treatment
Pre-treatment with Unmasking Fluid H (Art. No. DE008) at 96-100 C improves the staining results remarkably. For cryostat material acetone fixation (ice cold!) is recommended.

Immunogen
Recombinant human Uvomorulin (Cadherin-E) aa 600-707

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002.

Working Concentration
(liquid conc.) 1:10 - 1:50

Concentration
50 µg/ml

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002.

Applications
IHC(C, P)

Cross Reactivity
Human

Storage
2-8°C

References
1. Frixen U.H., et al. (1991) E-Cadherin-mediated cell-cell adhesion prevents invasivness of human carcinoma cells. J. Cell Biol. 113 ff. 2. Umbas R. Schalken J.A., Aalders T.W., Carter B.S., Karthaus H.F.M., Schaafsma H.E., Debruyne F.M.J., and Isaacs W.B. (1992) Expression of the cellular adhesion molecule E-Cadherin is reduced or absent in high-grade prostate cancer. Cancer Res. 52; 5104-5109. 3. Darai e., Scoazec J.Y., Walker-Combrouze F., Mlika-Cabanne N., Feldmann G., Madelenat P., and F. Potet (1997) Expression of cadherins in benign, borderline, and malignant ovarian epithelial tumors: a clinicopathologic study of 60 cases. Hum. Pathol. 28(8); 922-928. 4. Ruijter E., van de Kaa C., Aalders T. Ruiter D., Miller G., Debruyne F., and Schalken J. (1998) Heterogeneous expression of E-Cadherin and p53 in prostate cancer: clinical implications. BIOMED-II markers for prostate cancer study group. Mod. Pathol. 11(3); 276-281.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

E-Cadherin (Uvomorulin)

$632.00

SKU: CA150 Categories: ,