Collagen Type III, humanCatalogue number: CO20311-0.1
Type III collagen is an alpha1(III)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type III 100% bindung in RIA at 1:40 dilution, cross-reactivity with human collagen type I and IV <1.0%; human collagen type II and V <0.1%; human fibronectin and human laminin <0.1%.
Immunogen: Purified collagen type III from human placenta
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Purification Method: affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Concentration: app. 1 mg/ml
Secondary Reagents: Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488).
Species Reactivity: Human strictly, no cross-reactivity with other species so far tested (cattle, swine, rat, mouse)
IHC(P), IFA, ELISA, RIA, IB/WB
Incubation Time: IHC(P) 60 min at RT or 2-8°C over night
Working Concentration: (purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)
Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Positive Control: Human skin
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Black A.F., Bouez C., Perrier E., Schlotmann K., Chapuis F., Damour O. (2005) Optimization and characterization of an Engineered human skin equivalent. Tissue Engineering 11, 723-733. 2. Castera L., Hartmann D.J., Chapel F., Guettier C., Mal F., Lons T., Richardet J.P., Grimbert S., Morassi O., Beaugrand M., Trinchet J.C. (2000) Serum laminin and type IV collagen are accurate markers of histologically severe alcoholic hepatitis in patient 3. Ricard-Blum S., Hartmann D.J., Esterre Ph. (1998) Monitoring of extracellular matrix metabolism and cross-linking in tissue, serum and urine of patients with chromoblastomycosis, a chronic skin fibrosis. Eur. J. Clin. Invest. 28, 748-754.
Database Name: UniProt
Accession number: P02461 (CO3A1_HUMAN)
Species Accession: Human
Collagen Type III, human
|Collagen Type III on human skin paraffin section. CO20311, DAB, hematoxylin|