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Collagen Substrate, FITC Conjugate

Catalogue number: X1099

Product Type Purified Protein
Units10 mg
HostBovine
Application Substrate

Background
FITC-labeled bovine type II collagen substrate is an excellent substrate for examining collagenase activity. This labelled substrate is highly purified and telo-peptide free and is supplied as solution in acetic acid. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography. FITC-labeled collagen can also be used as a substrate for cell culture. Collagen degradation products in the culture supernatants can be directly determined by measured the flourescence at 520 nm (Emission)/490 nm (Excitation).

Synonyms: Extracted from bovine articular cartilage

Source

Immunogen: Bovine articular cartilage

Product

Product Form: FITC

Formulation: Provided as a 1 mg/ml solution in 0.01M acetic acid.

Purification Method: DEAE Anion Exchange Chromatography

Purity: >95%

Concentration: See vial for concentration

Applications
Used for the assay of mammalian collagenase activity.

Functional Analysis: Activity Assay

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Stable in acidic buffer when stored at -20OC, however slowly degrades when stored in under neutral conditions.

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

References
1. Terato, K., et al. 'A rapid assay method of collagenase activity using 14C-labeled soluble collagen as substrate.' Biochim. Biophys. Acta 1976, 445, 753-762 2. Sellers, A., et al. 'Evidence that latent collagenases are enzyme-inhibitor complexes.' Biochem. J. 1977, 163, 303-307 3. Shinkai, H., et al. 'A complex of collagenase with low molecular weight inhibitors in the culture medium of embryonic chick skin explants.' J. Biochem. (Tokyo) 1977, 81, 261-263 4. Shinkai, H. & Nagai, Y., 'A latent collagenase from embryonic human skin explants.' J. Biochem. (Tokyo) 1977, 81, 1261-1263 5. Murawaki, Y., et al. 'Serum collagenase activity in patients with chronic liver disease.' J. Hepatol. 1993, 328-334


Collagen Substrate, FITC Conjugate

$580.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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