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CML (N-Epsilon)-Carboxymethyl-Lysine


Catalogue number: CML024

CloneNF-1G
IsotypeIgG2a
Units50 µg
HostMouse
Application ELISA
IHC
Immunohistochemistry
Background
This antibody is suitable for the detection of CML in tissues and tissue extracts. Long-term incubation of proteins with glucose leads, through Schiff's base and Amadori rearrangement products, to the formation of advanced glycation end products (AGE) which are characterized by fluorescence, brown color and inter- and intra-molecular cross-linking. Recent immunological studies using anti-AGE antibodies demonstrated the presence of AGE in (i) human lens, (ii) renal proximal tubules in patients with diabetic nephropathy and chronic renal failure, (iii) atherosclerotic lesions of arterial walls, (iv) ß2-microglobulin of carpal tunnel amyloid fibril deposits in patients with hemodialysis-related amyloidosis and (v) brain tissues of patients with Alzheimer’s disease. N-epsilon-(carboxymethyl)-lysine was identified to be a major structure in AGE (Dunn et al., 1989). Oxidative cleavage of Amadori-products seems to be the main pathway of CML-formation in vivo. More recent investigations however have shown, that as well lipid-peroxidation as glycoxidation can be involved in CML-build up in vivo (Fu et al., 1996).

Immunogen
N-Epsilon-(Carboxymethyl)-Lysine (CML)-HSA

Purification Method
Protein G affinity purified antibody from ascites in stabilized buffer, containing 50% Block Ace? (Casein-containing solution, Dainippon Co.) and 0.1% ProClin? (Rohm & Haas) as a preservative

Working Concentration
(liquid conc.) ELISA 0.1-0.5 µg/ml; IHC 2 µg/ml

Concentration
0.25 mg/ml

Incubation Time
60 min at RT or 18 hr at 2-8°C

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005).

Product
Protein G affinity purified antibody from ascites in stabilized buffer, containing 50% Block Ace? (Casein-containing solution, Dainippon Co.) and 0.1% ProClin? (Rohm & Haas) as a preservative

Applications
IHC, ELISA

Storage
-20°C

References
1. Horiuchi S., Araki N., and Morino Y. (1991) Immunological approach to characterize advanced glycation end products of the Maillard reaction: Evidence for the presence of a common stucture. J. Biol. Chem. 266; 7329-7332. 2. Dunn J.A., Patrick J.S., Thorpe S.R., Baynes J.W. (1989): Oxidation of glycated proteins: age-dependent accumulation of N-epsilon-(carboxymethyl) lysine in lens proteins. Biochemistry. 28: 9464-9468. 3. Fu M.X., Requena J.R., Jenkins A.J., Lions T.J., Baynes J.W., Thorpe S.R. (1996): The advanced glycation end product, N-epsilon-(carboxymethyl) lysine, is a product of both lipid peroxidation and glycoxidation reactions. J.Biol.Chem.271: 9982-9986

Caution
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Safety Datasheet(s) for this product:

CML (N-Epsilon)-Carboxymethyl-Lysine

$1,059.00

SKU: CML024 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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